High expression and nuclear localization of β-catenin in diffuse large B-cell lymphoma
High expression and nuclear localization of β-catenin in diffuse large B-cell lymphoma
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DOI：
10.3892/mmr.2012.835
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发表时间：
2012-06-01
影响因子：
3.4
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Ge, Xueling;Lv, Xiao;Wang, Xin
Wnt/ β -连环蛋白信号通路在胚胎发育和成人器官和组织的维持以及一系列疾病（包括许多类型的癌症）的发病机制中发挥着多种作用。β -catenin是Wnt/ β -catenin通路的主要下游效应物，它迁移到细胞核并介导Wnt/ β -catenin通路的激活。本研究旨在探讨β -连环蛋白在弥漫大b细胞淋巴瘤（DLBCL）组织中的表达和定位，并阐明β -连环蛋白在DLBCL发病机制中的作用。采用定量聚合酶链反应（PCR）检测β -catenin mRNA表达水平，western blot和免疫组织化学染色检测β -catenin蛋白表达水平。与淋巴结组织反应性增生相比，DLBCL在mRNA和蛋白水平上均表现出更高的β -连环蛋白表达(P
The Wnt/beta-catenin signaling pathway plays diverse roles in embryonic development and maintenance of organs and tissues in adults as well as in the pathogenesis of a range of diseases, including many types of carcinomas. beta-catenin, the principal downstream effector of the Wnt/beta-catenin pathway, migrates to the nucleus and mediates the activation of the Wnt/beta-catenin pathway. The aim of the present study was to investigate the expression and localization of beta-catenin in diffuse large B-cell lymphoma (DLBCL) tissues and to illuminate the role of beta-catenin in the pathogenesis of DLBCL. The mRNA expression levels of beta-catenin were determined by quantitative polymerase chain reaction (PCR), while beta-catenin protein levels were detected by western blot analysis and immunohistochemical staining. DLBCL showed a higher expression of beta-catenin in contrast to reactive hyperplasia of lymph node tissues at both the mRNA and protein levels (P