Bioresorbable nanofiber-based systems for wound healing and drug delivery: Optimization of fabrication parameters
Bioresorbable nanofiber-based systems for wound healing and drug delivery: Optimization of fabrication parameters
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DOI：
10.1002/jbm.b.30041
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发表时间：
2004-08-15
影响因子：
3.4
通讯作者：
Laurencin, CT
中科院分区：
文献类型：
--
作者：
Katti, DS;Robinson, KW;Laurencin, CT
伤口愈合是一个复杂的过程，通常需要使用抗生素治疗。本文报道了基于可生物降解聚合物纳米纤维的抗生素递送系统的初步开发。这种系统的功能是（a）用作可生物降解的纱布，和（B）用作抗生素递送系统。在这项研究中使用的聚合物是聚（丙交酯-共-乙交酯）（PLAGA），和PLAGA的纳米纤维制造与使用的静电纺丝过程。本研究的目的是确定制造参数的影响：孔直径（针规），聚合物溶液浓度，和电压每单位长度，对静电纺纳米纤维的形态和直径。所研究的针规格为16（1.19 mm）、18（0.84 mm）和20（0.58 mm），并且所研究的聚合物溶液浓度范围为0.10 g/mL至0.30 g/mL。通过改变每单位静电纺丝距离的电压来确定电压的影响，并且所研究的范围为0.375 kV/cm至1.5 kV/cm。此外，测定了静电纺纳米纤维的单位面积质量随时间的变化，并研究了抗生素（头孢唑林）负载到纳米纤维中的可行性。结果表明，纳米纤维的直径随着针距的增加（孔直径的减小）而减小，并且随着聚合物溶液的浓度的增加而增加。电压研究表明，纳米纤维的平均直径随着电压的增加而减小。然而，与聚合物溶液浓度相比，电压对纤维直径的影响不太明显。面密度的研究结果表明，电纺纳米纤维的单位面积的质量随时间线性增加。使用头孢唑啉（一种广谱抗生素）证明了药物掺入纳米纤维的可行性。总之，这些研究表明，PLAGA纳米纤维可以通过修改加工参数来定制为所需的直径，并且抗生素如头孢唑啉可以掺入这些纳米纤维中。因此，PLAGA纳米纤维显示出作为用于治疗伤口的抗生素递送系统的潜力。(C)2004 Wiley Periodicals，Inc.
Wound healing is a complex process that often requires treatment with antibiotics. This article reports the initial development of a biodegradable polymeric nanofiber-based antibiotic delivery system. The functions of such a system would be (a) to serve as a biodegradable gauze, and (b) to serve as an antibiotic delivery system. The polymer used in this study was poly(lactide-co-glycolide) (PLAGA), and nanofibers of PLAGA were fabricated with the use of the electrospinning process. The objective of this study was to determine the effect of fabrication parameters: orifice diameter (needle gauge), polymer solution concentration, and voltage per unit length, on the morphology and diameter of electrospun nanofibers. The needle gauges studied were 16 (1.19 mm), 18 (0.84 mm), and 20 (0.58 mm), and the range of polymer solution concentration studied was from 0.10 g/mL to 0.30 g/mL. The effect of voltage was determined by varying the voltage per unit electrospinning distance, and the range studied was from 0.375 kV/cm to 1.5 kV/cm. In addition, the mass per unit area of the electrospun nanofibers as a function of time was determined and the feasibility of antibiotic (cefazolin) loading into the nanofibers was also studied. The results indicate that the diameter of nanofibers decreased with an increase in needle gauge (decrease in orifice diameter), and increased with an increase in the concentration of the polymer solution. The voltage study demonstrated that the average diameter of the nanofibers decreased with an increase in voltage. However, the effect of voltage on fiber diameter was less pronounced as compared to polymer solution concentration. The results of the areal density study indicated that the mass per unit area of the electrospun nanofibers increased linearly with time. Feasibility of drug incorporation into the nanofibers was demonstrated with the use of cefazolin, a broad-spectrum antibiotic. Overall, these studies demonstrated that PLAGA nanofibers can be tailored to desired diameters through modifications in processing parameters, and that antibiotics such as cefazolin can be incorporated into these nanofibers. Therefore, PLAGA nanofibers show potential as antibiotic delivery systems for the treatment of wounds. (C) 2004 Wiley Periodicals, Inc.