Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses
Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses
复制标题
环境胁迫下水稻类钙调神经磷酸酶 B 基因家族的表达分析
DOI：
10.1016/j.gene.2008.02.011
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发表时间：
2008-05-31
期刊：
影响因子：
3.5
通讯作者：
Zhang, Hongsheng
中科院分区：
文献类型：
--
作者：
Gu, Zhimin;Ma, Bojun;Zhang, Hongsheng
钙作为第二信使在环境胁迫下介导各种防御反应中起着至关重要的作用。钙调神经磷酸酶B样蛋白（CBL）是植物特异性钙信号转导中的重要钙感受器。根据序列的相似性，通过检索粳稻基因组数据库，鉴定出10个CBL基因，随机分布在第1、2、3、5、10和12号染色体上。通过半定量RT-PCR方法检测了各基因在成株期各器官和经NaCl、PEG、低温胁迫或外源阿坝处理的幼苗中的表达模式。结果表明，各水稻CBL基因的诱导不仅对不同的胁迫条件有反应，而且具有器官特异性。体内靶向实验表明OsCBL 8定位于细胞质膜，这与之前报道的OsCBL 4和SOS 3一致。为了阐明OsCBL 8基因的功能，通过农杆菌介导的方法获得了过量表达OsCBL 8基因的水稻植株。OsCBL 8转基因水稻幼苗表现出比非转基因幼苗更强的耐盐性。(C)2008 Elsevier B. V.保留所有权利。
Calcium plays a crucial role as a second messenger in mediating various defense responses under environmental stresses. Calcineurin B-like (CBL) proteins have been implicated as important Ca2+ sensors in plant-specific calcium signaling. Based on the similarity of sequence, ten CBL genes were identified by searching the rice japonica genome database, which were randomly distribute on chromosomes 1, 2, 3, 5, 10 and 12. By semi-quantitative RT-PCR approach the expression pattern of each gene was detected in various organs at the adult stage and seedlings treated with NaCl, PEG and cold stresses or exogenous ABA. The results showed that the induction of each rice CBL gene was not only responsive to different stress conditions, but also organ specific. In vivo targeting experiment revealed that OsCBL8 localized to the plasma membrane, which was consistent with OsCBL4 and SOS3 previously reported. To elucidate the putative function of OsCBL8 gene, transgenic rice plants overexpressing OsCBL8 gene were generated by the Agrobacterium-mediated approach. The OsCBL8 transgenic rice seedlings showed more tolerance to salt stress than non-transgenic seedlings. (C) 2008 Elsevier B.V. All rights reserved.