A Core-Shell-Satellite Structured Fe3O4@g-C3N4-UCNPs-PEG for T1/T2-Weighted Dual-Modal MRI-Guided Photodynamic Therapy
A Core-Shell-Satellite Structured Fe3O4@g-C3N4-UCNPs-PEG for T1/T2-Weighted Dual-Modal MRI-Guided Photodynamic Therapy
复制标题
用于 T-1/T-2 加权双模态 MRI 引导光动力治疗的核壳卫星结构 Fe3O4@g-C3N4-UCNPs-PEG
DOI：
10.1002/adhm.201700502
复制
发表时间：
2017-09-20
影响因子：
10
通讯作者：
Yang, Piaoping
中科院分区：
文献类型：
--
作者：
Feng, Lili;Yang, Dan;Yang, Piaoping
特定肿瘤部位产生的活性氧（ROS）在光动力疗法（PDT）中发挥着关键作用。在此，通过在介孔石墨相氮化碳（g-C3N4）涂覆的超顺磁性氧化铁纳米球上吸收超小型上转换纳米粒子（UCNP），然后用聚乙二醇（PEG）分子（缩写为Fe3O4@g-C3N4-UCNPs-PEG）进一步修饰，设计了多功能纳米平台。 Fe3O4 核心和外部 UCNP 之间的惰性 g-C3N4 层可以显着抑制 Fe3O4 对上转换发射的猝灭效应。在近红外（NIR）激光照射下，UCNPs通过荧光共振能量转移过程将能量转换到光敏剂（g-C3N4层），从而产生大量的ROS。由于纳米颗粒的细胞摄取和有效的 PDT 功效，体外实验表现出明显的近红外触发细胞抑制作用。值得注意的是，该平台对磁场敏感，能够在外部磁场的引导下进行靶向递送，并通过T-1/T-2加权双模态磁共振成像来监督治疗效果。此外，体内治疗效果表明，磁性引导的Fe3O4@g-C3N4-UCNPs-PEG的积累几乎可以触发完全的肿瘤抑制，而没有任何可察觉的副作用。实验强调了Fe3O4@g-C3N4-UCNPs-PEG作为PDT应用的磁性靶向平台的良好前景。
Reactive oxygen species (ROS) produced in the specific tumor site plays the key role in photodynamic therapy (PDT). Herein, a multifunctional nanoplatform is designed by absorbing ultrasmall upconversion nanoparticles (UCNPs) on mesoporous graphitic-phase carbon nitride (g-C3N4) coated superparamagnetic iron oxide nanospheres, then further modified with polyethylene glycol (PEG)molecules (abbreviated as Fe3O4@g-C3N4-UCNPs-PEG). The inert g-C3N4 layer between Fe3O4 core and outer UCNPs can substantially depress the quenching effect of Fe3O4 on the upconversion emission. Upon near-infrared (NIR) laser irradiation, the UCNPs convert the energy to the photosensitizer (g-C3N4 layer) through fluorescence resonance energy transfer process, thus producing a vast amount of ROS. In vitro experiment exhibits an obvious NIR-triggered cell inhibition due to the cellular uptake of nanoparticles and the effective PDT efficacy. Notably, this platform is responsive to magnetic field, which enables targeted delivery under the guidance of an external magnetic field and supervises the therapeutic effect by T-1/T-2-weighted dual-modal magnetic resonance imaging. Moreover, in vivo therapeutic effect reveals that the magnetism guided accumulation of Fe3O4@g-C3N4-UCNPs-PEG can almost trigger a complete tumor inhibition without any perceived side effects. The experiments emphasize that the excellent prospect of Fe3O4@g-C3N4-UCNPs-PEG as a magnetic targeted platform for PDT application.