The enzymic synthesis of aryl sulphamates.
The enzymic synthesis of aryl sulphamates.
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芳基氨基磺酸盐的酶法合成。
DOI：
10.1042/bj0740049
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发表时间：
1958
期刊：
影响因子：
--
通讯作者：
A. Roy
中科院分区：
文献类型：
--
作者：
A. Roy
Boland，Manson&Orr(1957)已经证明，给大鼠或兔子注射某些芳胺之后，相应的芳基磺酸酯类化合物会在尿液中排泄，这些化合物以前没有被认识到自然存在。随后展示了用大鼠肝脏制剂(Roy，1958)和绵羊肠道粘膜酶(Kent&Pasternak，1958)体外合成2-萘基氨基甲酸酯(Roy，1958)。这些结果令人感兴趣，因为所涉及的反应可能与那些参与合成肝素的磺酸基的反应有关(Foster&Huggard，1955)。根据目前关于硫酸酯合成的想法(Lipmann，1958)，芳基磺酸酯的合成似乎可能涉及到从活性硫酸盐-3‘-腺苷-磷酸-5’-硫酸盐的硫酸盐转移，这一反应是由一种特殊的硫酸激酶--芳胺硫酸激酶催化的。本文研究了芳基磺酸酯的酶促合成，特别是某些甾体化合物对该反应的影响。这项工作的初步记述已经出现(Roy，1959)。
Boyland, Manson & Orr (1957) have shown that the administration of certain arylamines to rats or to rabbits is followed by the urinary excretion of the corresponding aryl sulphamates, compounds whose natural occurrence had not previously been recognized. This was followed by the demonstration of the synthesis in vitro of 2-naphthyl sulphamate by rat-liver preparations (Roy, 1958) and of phenyl sulphamate by sheep intestinal-mucosal enzymes (Kent & Pasternak, 1958). These results were of interest, as the reactions involved might be expected to be related to those involved in the synthesis of the sulphamate groups of heparin (Foster & Huggard, 1955). In the light of the present ideas on the synthesis of sulphuric acid esters (Lipmann, 1958) it seemed likely that the synthesis of aryl sulphamates would involve sul-phate transfer from'active sulphate', adenosine 3'-phosphate 5'-sulphatophosphate, this reaction being catalysed by a specific sulphokinase, arylamine sulphokinase. The present paper describes an investigation into the enzymic synthesis of aryl sulphamates and, in particular, into theinfluence of certain steroids on this reaction. A preliminary account of the work has already appeared (Roy, 1959).