Astaxanthin, a carotenoid without vitamin A activity, augments antibody responses in cultures including T-helper cell clones and suboptimal doses of antigen.
Astaxanthin, a carotenoid without vitamin A activity, augments antibody responses in cultures including T-helper cell clones and suboptimal doses of antigen.
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DOI：
10.1093/jn/125.10.2483
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发表时间：
1995-10
期刊：
影响因子：
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通讯作者：
H. Jyonouchi;S. Sun;Y. Tomita;M. Gross
中科院分区：
文献类型：
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作者：
H. Jyonouchi;S. Sun;Y. Tomita;M. Gross
虾青素是一种没有维生素a活性的类胡萝卜素，可增强t依赖性抗原（Ag）特异性体液免疫反应。我们通过重构实验直接检测了类胡萝卜素对辅助性t细胞活性的影响；脾Th细胞用ag特异性的1型和2型（Th1和Th2） Th细胞克隆代替。Th1和Th2克隆的抗原分别为鸽细胞色素C和兔γ -球蛋白。虾青素和β -胡萝卜素增加了未引物的B细胞与Th克隆孵育，并对每个Th克隆进行次优剂量的Ag特异性刺激，从而增加了IgM抗体（Ab）分泌细胞的数量。在这两个克隆中，使用体内引物的B细胞比使用未引物的B细胞分泌IgG抗体的细胞数量更多。虾青素而非β -胡萝卜素增加了IgG抗体分泌细胞的数量，当启动的B细胞和Th细胞克隆受到次优剂量的Ag特异性刺激时。在每个Th克隆中存在最佳剂量的Ag时，类胡萝卜素都没有增加ab分泌细胞的数量。虾青素和β -胡萝卜素可以增强Th1和Th2细胞在次优银挑战时的体液免疫反应；某些类胡萝卜素可能有助于将ag介导的免疫反应维持在最佳水平。
Astaxanthin, a carotenoid without vitamin A activity, enhances T-dependent antigen (Ag)-specific humoral immune responses. We examined carotenoid actions on T-helper (Th) cell activity in a direct manner with reconstitution experiments; spleen Th cells were replaced with Ag-specific Type 1 and Type 2 (Th1 and Th2) Th cell clones. The Ag for the Th1 and Th2 clones were pigeon cytochrome C and rabbit gamma-globulin, respectively. Astaxanthin and beta-carotene augmented the number of IgM antibody (Ab)-secreting cells when unprimed B cells were incubated with Th clones and stimulated with suboptimal doses of Ag specific for each Th clone. The number of IgG Ab-secreting cells were greater with use of in vivo primed B cells than with unprimed B cells in both Th clones. Astaxanthin but not beta-carotene augmented the number of IgG Ab-secreting cells when primed B cells and Th cell clones were stimulated with suboptimal doses of Ag specific for each Th clone. In the presence of optimal doses of Ag for each Th clone, neither carotenoid augmented the number of Ab-secreting cells. Astaxanthin and beta-carotene may enhance the actions of both Th1 and Th2 cells for humoral immune responses with suboptimal Ag challenges; certain carotenoids may help maintain Ag-mediated immune responses at optimal levels.