Killing by Bactericidal Antibiotics Does Not Depend on Reactive Oxygen Species
Killing by Bactericidal Antibiotics Does Not Depend on Reactive Oxygen Species
复制标题
DOI：
10.1126/science.1232688
复制
发表时间：
2013-03-08
期刊：
影响因子：
56.9
通讯作者：
Lewis, Kim
中科院分区：
文献类型：
--
作者：
Keren, Iris;Wu, Yanxia;Lewis, Kim
杀菌抗生素通过调节它们各自的靶标来杀死细菌。这一传统观点受到了一些研究的挑战，这些研究提出了一种替代的、统一的杀戮机制，即在抗生素存在的情况下产生有毒的活性氧(ROS)。我们发现单个细胞在抗生素存在下存活的概率与其ROS水平之间没有相关性。一种名为硫脲的ROS猝灭剂保护细胞免受低浓度抗生素的影响，但在厌氧条件下也观察到了这种效果。在好氧或厌氧条件下，不同抗生素处理的细菌的存活率基本上没有差异。这表明，ROS在抗生素杀死细菌病原体方面没有发挥作用。
Bactericidal antibiotics kill by modulating their respective targets. This traditional view has been challenged by studies that propose an alternative, unified mechanism of killing, whereby toxic reactive oxygen species (ROS) are produced in the presence of antibiotics. We found no correlation between an individual cell's probability of survival in the presence of antibiotic and its level of ROS. An ROS quencher, thiourea, protected cells from antibiotics present at low concentrations, but the effect was observed under anaerobic conditions as well. There was essentially no difference in survival of bacteria treated with various antibiotics under aerobic or anaerobic conditions. This suggests that ROS do not play a role in killing of bacterial pathogens by antibiotics.