Heparan sulfate in the stone matrix and its inhibitory effect on calcium oxalate crystallization
Heparan sulfate in the stone matrix and its inhibitory effect on calcium oxalate crystallization
复制标题
结石基质中的硫酸乙酰肝素及其对草酸钙结晶的抑制作用
DOI：
10.1007/bf00590035
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发表时间：
1993
影响因子：
--
通讯作者：
T. Sonoda
中科院分区：
文献类型：
--
作者：
S. Yamaguchi;T. Yoshioka;M. Utsunomiya;T. Koide;M. Osafune;A. Okuyama;T. Sonoda
本文研究了可溶性结石基质的性质及其在泌尿系结石形成中的可能作用。为此，我们进行了二维醋酸纤维素膜电泳的糖胺聚糖（GAG），其中所含的可溶性结石基质，物质吸附到草酸钙晶体在体外（晶体表面结合物质，CSBS）和尿大分子（UMMs）。发现可溶性结石基质和CSBS中的主要GAG是硫酸乙酰肝素，而UMM含有通常在尿液中观察到的各种GAG。抑制试验表明，当抑制活性以糖醛酸浓度表示时，可溶性结石基质在这些大分子物质中具有最强的抑制活性。这表明可溶性结石基质中的主要GAG由硫酸乙酰肝素组成，硫酸乙酰肝素对草酸钙晶体生长和聚集具有较强的抑制活性，并构成CSBS的一部分。
SummaryThe nature of the soluble stone matrix and its possible role in urinary stone formation was studied. For this purpose we performed two-dimensional cellulose acetate membrane electrophoresis of the glycosaminoglycans (GAGs) which were contained in the soluble stone matrix, substances adsorbed onto calcium oxalate crystals in vitro (crystal surface binding substances, CSBS) and urinary macromolecules (UMMs). The main GAG in the soluble stone matrix and CSBS was found to be heparan sulfate, whereas the UMMs contained various GAGs usually seen in urine. An inhibition assay showed the soluble stone matrix to have the strongest inhibitory activity among these macromolecular substances when inhibitory activity was expressed in terms of uronic acid concentration. It is suggested that the main GAG in the soluble stone matrix consists of heparan sulfate, which has a strong inhibitory activity on calcium oxalate crystal growth and aggregation and constitutes part of the CSBS.