HIGH EXPRESSION OF STATHMIN IN MULTIPOTENTIAL TERATOCARCINOMA AND NORMAL EMBRYONIC-CELLS VERSUS THEIR EARLY DIFFERENTIATED DERIVATIVES
HIGH EXPRESSION OF STATHMIN IN MULTIPOTENTIAL TERATOCARCINOMA AND NORMAL EMBRYONIC-CELLS VERSUS THEIR EARLY DIFFERENTIATED DERIVATIVES
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DOI：
10.1111/j.1432-0436.1992.tb00489.x
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发表时间：
1992-06-01
期刊：
影响因子：
2.9
通讯作者：
SOBEL, A
中科院分区：
文献类型：
--
作者：
DOYE, V;KELLERMANN, O;SOBEL, A
Stathmin是一种普遍存在的细胞质蛋白，在调节细胞增殖、分化和特化功能的药物的作用下被磷酸化，在某种程度上可能整合了多种伴随的调节信号的作用。它的表达也与细胞增殖和分化有关，并在新生儿期达到高峰。为了评估安定素在早期发育阶段的可能作用，我们检测了其在胚胎癌（EC）及其衍生细胞系以及早期小鼠胚胎中的表达和调控。有趣的是，安定素在F9、1003和1009 EC细胞系的未分化多能细胞中含量很高。当视黄酸和二丁基camp诱导F9细胞分化为内胚层样细胞时，其高表达量在蛋白和mRNA水平上均显著降低。在分化的细胞系中，如滋养外胚层细胞系TDM-1，以及一些F9和1003衍生的细胞系，它们致力于向中胚层和神经外胚层细胞系分化，但仍在增殖，Stathmin的含量也要少得多。因此，观察到的stathmin表达的降低与增殖速率的降低无关，而与多能性EC细胞的分化有关。小鼠早期发育过程中安定素表达的免疫细胞化学模式表明，安定素在囊胚内细胞群的多能细胞中表达量也很高，而在分化的滋养外胚层细胞中表达量要低得多。这些结果证实了观察结果与EC细胞的生理学相关性，并表明除了在发育后期和成年神经系统中高表达外，安定素可能被认为是早期小鼠胚胎多电位细胞的新标记物。
Stathmin is a ubiquitous cytoplasmic protein, phosphorylated in response to agents regulating the proliferation, the differentiation and the specialized functions of cells, in a way possibly integrating the actions of diverse concomitant regulatory signals. Its expression is also regulated in relation with cell proliferation and differentiation and reaches a peak at the neonatal stage.To assess the possible role of stathmin at earlier stages of development, we examined its expression and regulation in embryonal carcinoma (EC) and derived cell lines as well as in the early mouse embryo. Interestingly, stathmin is highly abundant in the undifferentiated, multipotential cells of the F9, 1003 and 1009 EC cell lines. Its high expression markedly decreased, both at the protein and mRNA levels, when F9 cells were induced to differentiate into endodermal-like cells with retinoic acid and dibutyryl-cAMP. Stathmin was also much less abundant in differentiated cell lines such as the trophectodermal line TDM-1, as well as in several F9- and 1003-derived cell lines commited to differentiate towards the mesodermal and neuroectodermal lineages but still proliferating. Therefore, the observed decrease of stathmin expression is not related to the reduced proliferation rate but rather to the differentiation of the multipotential EC cells.The immunocytochemical pattern of stathmin expression during early mouse development indicated that stathmin is also highly abundant in the multipotential cells of the inner cell mass of the blastula, whereas it is much lower in the differentiated trophectodermal cells. These results confirm the physiological relevance of the observations with EC cells, and suggest that stathmin, in addition to its high expression at later stages of development and in the adult nervous system, may be considered as a new marker of the multipotential cells of the early mouse embryo.