Genetic diversity and differentiation of Quercus semiserrata Roxb. in northern Thailand revealed by nuclear and chloroplast microsatellite markers
Genetic diversity and differentiation of Quercus semiserrata Roxb. in northern Thailand revealed by nuclear and chloroplast microsatellite markers
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DOI：
10.1016/j.foreco.2007.10.021
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发表时间：
2008-03
影响因子：
3.7
通讯作者：
Greuk Pakkad;S. Ueno;H. Yoshimaru
中科院分区：
文献类型：
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作者：
Greuk Pakkad;S. Ueno;H. Yoshimaru
本研究描述了 10 个半锯齿栎种群的遗传多样性和分化水平。在泰国北部，根据地理位置分为四组（A-D）。检查了八个核 (nSSR) 和九个叶绿体 (cpSSR) 微卫星位点的变异。使用的 8 个核微卫星位点总共检测到 139 个等位基因 (n=392)，并根据 9 个 cpSSR 位点的长度变异鉴定出 16 个单倍型。 B组人群的nSSR和cpSSR均具有最高的基因多样性（H）和等位基因丰富度（Ar）（nSSR，H=0.79和Ar=7.3；cpSSR，H=0.5和Ar=2.5），C组人群的nSSR具有最低的基因多样性和等位基因丰富度，而A组人群的cpSSR具有最低的基因多样性和等位基因丰富度（Ar）。单倍型丰富度。对每个种群对总多样性和等位基因丰富度的贡献的计算（AMOVA）表明，nSSR 变异的大部分（86.62%）是由于种群内的差异造成的，但叶绿体单倍型的总遗传变异的大部分（63.72%）是由于群体内种群之间的差异造成的。花粉流与种子流的比率估计为 94:1。对于 cpSSR，成对 FST/(1−FST) 值与人群之间的地理距离显着相关（r=0.337；P<0.05），但与 nSSR 无关。构建了基于 nSSR Nei 遗传距离的 UPGMA 和 NJ 系统发育树。结果表明，B 组中的四个种群（Khun Wang、Oblang、Doi Suthep 和 Doi Inthanon）具有最高的遗传多样性和大量的叶绿体单倍型。因此，这些种群应该成为该物种保护的最高优先级。这是第一份关于该物种遗传多样性和分化的报告，并提供了有助于保护该物种的基本遗传信息。
This study describes the level of genetic diversity and differentiation of 10 populations of Quercus semiserrata Roxb. in northern Thailand, divided into four groups (A–D) based on their geographical location. Variation at eight nuclear (nSSR) and nine chloroplast (cpSSR) microsatellite loci was examined. The eight nuclear microsatellite loci employed detected a total of 139 alleles (n=392), and 16 haplotypes based on length variants at the nine cpSSR loci were identified. Populations in group B harbored the highest gene diversity (H) and allelic richness (Ar) in both their nSSRs and cpSSRs (nSSR, H=0.79 and Ar=7.3; cpSSR, H=0.5 and Ar=2.5), the nSSRs of populations in group C had the lowest gene diversity and allelic richness, while the cpSSRs of populations in group A had the lowest gene diversity and haplotypic richness. Calculations (AMOVA) of each population's contributions to the total diversity and allelic richness indicated that most (86.62%) of the variation in nSSRs was due to differences within populations, but most (63.72%) of the total genetic variation in chloroplast haplotypes was due to differences among populations within groups. The ratio of pollen flow to seed flow was estimated to be 94:1. Pairwise FST/(1−FST) values were significantly correlated with geographic distances among populations for cpSSRs (r=0.337; P<0.05), but not nSSRs. UPGMA and NJ phylogenetic trees based on Nei's genetic distances for nSSRs were constructed. The results suggest that four populations in group B (Khun Wang, Obluang, Doi Suthep and Doi Inthanon) had the highest genetic diversity and high numbers of chloroplast haplotypes. Therefore, these populations should be given the highest priority for conservation of this species. This is the first report concerning the genetic diversity and differentiation of this species, and provides basic genetic information that should facilitate attempts to conserve the species.