Identification of Protein Kinase Substrates by the Kinase-Interacting Substrate Screening (KISS) Approach.
Identification of Protein Kinase Substrates by the Kinase-Interacting Substrate Screening (KISS) Approach.
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DOI：
10.1002/cpcb.8
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发表时间：
2016-09-01
影响因子：
--
通讯作者：
Kaibuchi, Kozo
中科院分区：
文献类型：
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作者：
Amano, Mutsuki;Nishioka, Tomoki;Kaibuchi, Kozo
鉴定蛋白激酶的底物以了解其作用模式已经通过各种方法进行，并且仍然是一个持续的挑战。磷酸蛋白质组学技术加速了有关蛋白质磷酸化的数据积累，并发现了体内大量的磷酸化位点。在这个单元中，提出了一种新的蛋白激酶底物的体外筛选方法，基于蛋白质-蛋白质相互作用和基于质谱的磷酸化蛋白质组学技术。John Wiley & Sons，Inc.
Identifying the substrates of protein kinases to understand their modes of action has been undertaken by various approaches and remains an ongoing challenge. Phosphoproteomic technologies have accelerated the accumulation of data concerning protein phosphorylation and have uncovered vast numbers of phosphorylation sites in vivo. In this unit, a novel in vitro screening approach for protein kinase substrates is presented, based on protein-protein interaction and mass spectrometry-based phosphoproteomic technology. © 2016 by John Wiley & Sons, Inc.