Multidrug Resistance and Plasmid Patterns of Escherichia coli O157 and Other E-coli Isolated from Diarrhoeal Stools and Surface Waters from Some Selected Sources in Zaria, Nigeria
Multidrug Resistance and Plasmid Patterns of Escherichia coli O157 and Other E-coli Isolated from Diarrhoeal Stools and Surface Waters from Some Selected Sources in Zaria, Nigeria
复制标题
DOI：
10.3390/ijerph7103831
复制
发表时间：
2010-10-01
影响因子：
--
通讯作者：
Okoh, Anthony I.
中科院分区：
文献类型：
--
作者：
Chigor, Vincent N.;Umoh, Veronica J.;Okoh, Anthony I.
我们评估了尼日利亚扎里亚（Zaria）部分选定来源的腹泻患者和地表沃茨中大肠杆菌O 157的流行情况，评估了184株大肠杆菌的抗生素敏感性和质粒谱。大肠杆菌分离株，从228个水样和112个粪便标本（收集年龄< 15岁的儿童），使用标准方法。E.地面沃茨中O 157大肠杆菌检出率为2.2%，腹泻儿童中O 157大肠杆菌检出率为5.4%。抗菌药物中庆大霉素、氯霉素、氟喹诺酮类抗生素活性最高。184株E.大肠杆菌对4种或4种以上抗生素耐药。多药耐药（MDR）是高之间的水生分离株比临床分离株。在35株MDR分离株（其中20株为O 157株）中，22株（62.9%）携带质粒，且质粒大小均不小于2.1kb。在20个E。coliO 157菌株中，只有7株（35.0%）含有多质粒。一株含有两种质粒的O 157菌株对氨苄西林、头孢呋辛、环丙沙星、复方新诺明、萘啶酸、呋喃妥因和四环素等7种药物耐药。在四环素（50 μ g/mL）-营养琼脂平板中选择的治愈（突变）菌株中，质粒丢失与抗生素抗性丢失相关。我们的研究结果表明，质粒普遍存在于水生和临床分离株，并建议所观察到的MDR是质粒介导的。质粒介导的多药耐药大肠杆菌的发生。作为饮用、娱乐和新鲜农产品灌溉来源的地表沃茨中的大肠杆菌O 157加剧了公众健康问题。
We have assessed the prevalence of Escherichia coli O157 in diarrhoeal patients and surface waters from some selected sources in Zaria (Nigeria), evaluating the antibiotic susceptibility and plasmid profiles of 184 E. coli isolates, obtained from 228 water samples and 112 diarrhoeal stool specimens (collected from children aged < 15 years), using standard methods. The detection rate of E. coli O157 in surface waters was 2.2% and its prevalence in children with diarrhoea was 5.4%. The most active antibiotics were gentamicin, chloramphenicol and fluoroquinolones. Seventy-nine (42.9%) of 184 E. coli isolates were resistant to four or more antibiotics. Multidrug resistance (MDR) was higher amongst aquatic isolates than the clinical isolates. Out of 35 MDR isolates (20 of which were O157 strains), 22 (62.9%) harboured plasmids all of which were no less than 2.1 kb in size. Amongst the 20 E. coli O157 strains, only seven (35.0%) contained multiple plasmids. An aquatic O157 isolate containing two plasmids was resistant to seven drugs, including ampicillin, cefuroxime, ciprofloxacin, cotrimoxazole, nalidixic acid, nitrofurantoin and tetracycline. Loss of plasmid correlated with loss of resistance to antibiotics in cured (mutant) strains selected in tetracycline (50 mu g/mL)-nutrient agar plates. Our findings revealed that plasmids were prevalent in both the aquatic and clinical isolates, and suggest that the observed MDR is plasmid-mediated. The occurrence of plasmid-mediated multidrug resistant E. coli O157 in surface waters used as sources for drinking, recreation and fresh produce irrigation heightens public health concern.