QTL analysis of fusarium head blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties
QTL analysis of fusarium head blight severity in recombinant inbred population derived from a cross between two-rowed barley varieties
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DOI：
10.1270/jsbbs.56.25
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发表时间：
2006-03-01
期刊：
影响因子：
2.4
通讯作者：
Takeda, K
中科院分区：
文献类型：
--
作者：
Hori, K;Sato, K;Takeda, K
以两棱大麦品种哈尔滨二棱(抗病)与火鸡6号(感病)杂交的一组重组自交系为材料，对赤霉病的严重程度进行了鉴定。在冈山大学保存的约5000份大麦种质资源中，哈尔滨2-ROW是对赤霉病抗性最强的品种之一。通过2000-2001年和2003-2004年两个季节的“剪穗试验”，对RI品系及其亲本的FHB严重程度进行了评价。根据从抗性(0)到感病(10)的11个等级对有病害症状的穗进行评分。构建了一个包含328个AFLP、45个SSR、1个RFLP-STS、1个AFLP-STS、4个抗性基因类似物(RGA)和4个形态标记的高密度连锁图谱，用于检测赤霉病严重程度的QTL。利用复合区间作图法，在2000-2001季节检测到2H染色体上的一个QTL，在2003-2004年季节检测到4H染色体上的一个QTL和6H染色体上的另一个QTL。在这3个QTL上，来自哈尔滨2行的等位基因对严重程度的贡献较小。一个QTL油性染色体2H位于决定开花/闭花类型的闭花受精基因(cly1或cly2)附近。与作者已定位的赤霉病严重程度QTL的比较表明，哈尔滨2-ROW在cly1/cly2位点附近可能含有与先前研究中用作抗病亲本的俄罗斯6号相同的抗性等位基因。位于cly1/cly2位点附近的QTL是大麦抗赤霉病育种的重要来源之一。
Fusarium head blight (FHB) severity was evaluated in a set of recombinant inbred (RI) lines from a cross between the two-rowed barley varieties, Harbin 2-row (resistant) and Turkey 6 (susceptible). Harbin 2-row was one of the most resistant varieties to FHB among ca. 5,000 barley germplasm accessions stored at Okayama University. The FHB severity of the RI lines and their parents was evaluated by the 'cut-spike test' in the 2000-2001 and 2003-2004 seasons. Spikes with disease symptoms were scored based on eleven grades from resistant (0) to susceptible (10). A high-density linkage map, including 328 AFLP, 45 SSR, one RFLP-STS, one AFLP-STS, four resistance gene analog (RGA) and four morphological markers, was constructed to detect QTLs for FHB severity. Composite interval mapping enabled to detect one QTL on chromosome 2H in the 2000-2001 season, and one QTL on chromosome 4H and another QTL on chromosome 6H in the 2003-2004 season. At these three QTLs, the alleles from Harbin 2-row contributed to a lower severity. A QTL oil chromosome 2H was located in the vicinity of the cleistogamy locus (cly1 or Cly2) that determines open/closed flowering type. The comparison with previously identified QTLs for FHB severity by the present authors indicated that Harbin 2-row might harbor the same resistance allele in the vicinity of the cly1/Cly2 locus as Russia 6, which was used as a resistant parent in the previous study. The QTL in the vicinity of the cly1/Cly2 locus is one of the promising sources for FHB resistance breeding in barley.