ErpA, an iron-sulfur (Fe-S) protein of the A-type essential for respiratory metabolism in Escherichia coli
ErpA, an iron-sulfur (Fe-S) protein of the A-type essential for respiratory metabolism in Escherichia coli
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DOI：
10.1073/pnas.0705829104
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发表时间：
2007-08-21
影响因子：
11.1
通讯作者：
Barras, Frederic
中科院分区：
文献类型：
--
作者：
Loiseau, Laurent;Gerez, Catherine;Barras, Frederic
了解铁硫 (Fe-S) 蛋白的生物发生与许多领域相关，包括生物能学、基因调控和癌症研究。已在原核生物和真核生物中鉴定出几种有助于 Fe-S 组装的多蛋白复合物。在这里，我们在大肠杆菌中鉴定出一种 A 型 Fe-S 蛋白，我们将其命名为 ErpA。值得注意的是，erpA被发现对于大肠杆菌在氧气或替代电子受体存在下的生长至关重要。结论是erpA突变损害了类异戊二烯的生物合成。首先，真核甲羟戊酸依赖性异戊烯基二磷酸生物合成途径恢复了erpA突变体的呼吸缺陷。其次，erpA突变体所含的泛醌和甲基萘醌的量大大减少。第三，ErpA 结合 Fe-S 簇并将它们转移到 apo-IspG，这是一种在大肠杆菌中催化异戊烯基二磷酸生物合成的蛋白质。令人惊讶的是，erpA 基因的定位与任何其他 Fe-S 生物发生相关基因都有一定距离。 ErpA 是一种 A 型 Fe-S 蛋白，其特征是在细胞代谢中发挥重要作用。
Understanding the biogenesis of iron-sulfur (Fe-S) proteins is relevant to many fields, including bioenergetics, gene regulation, and cancer research. Several multiprotein complexes assisting Fe-S assembly have been identified in both prokaryotes and eukaryotes. Here, we identify in Escherichia coli an A-type Fe-S protein that we named ErpA. Remarkably, erpA was found essential for growth of E. coli in the presence of oxygen or alternative electron acceptors. It was concluded that isoprenoid biosynthesis was impaired by the erpA mutation. First, the eukaryotic mevalonate-dependent pathway for biosynthesis of isopentenyl diphosphate restored the respiratory defects of an erpA mutant. Second, the erpA mutant contained a greatly reduced amount of ubiquinone and menaquinone. Third, ErpA bound Fe-S clusters and transferred them to apo-IspG, a protein catalyzing isopentenyl diphosphate biosynthesis in E. coli. Surprisingly, the erpA gene maps at a distance from any other Fe-S biogenesis-related gene. ErpA is an A-type Fe-S protein that is characterized by an essential role in cellular metabolism.