Expression patterns of Xenopus FGF receptor-like 1/nou-darake in early Xenopus development resemble those of planarian nou-darake and Xenopus FGF8
Expression patterns of Xenopus FGF receptor-like 1/nou-darake in early Xenopus development resemble those of planarian nou-darake and Xenopus FGF8
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DOI：
10.1002/dvdy.20040
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发表时间：
2004-08-01
影响因子：
2.5
通讯作者：
Taira, M
中科院分区：
文献类型：
--
作者：
Hayashi, S;Itoh, M;Taira, M
成纤维细胞生长因子（FGF）在早期发育过程中介导许多细胞间信号传导事件。Nou-darake（ndk）是一个编码FGF受体（FGFR）样分子的基因，在日本三角涡虫（Dugesia japonica）头部区域特异性表达，其功能分析为FGF信号通路脑诱导回路的存在提供了有力的分子证据。为了分析ndk在脊椎动物发育过程中的作用，我们分离了ndk的非洲爪蟾直系同源物，脊椎动物FGFR样1基因（XFGFRL 1）。XFGFRL 1/Xndk的表达首先在原肠胚晚期的前部区域中检测到，并且在整个前中内胚层区域（包括前索板、近轴中胚层、前内胚层和原肠顶）的神经胚早期显著增加。这种前表达模式类似于在涡虫ndk，这表明FGFRL 1/ndk的表达是保守的进化这两个遥远的分歧生物。在尾芽期，XFGFRL 1/Xndk在前脑、眼、中脑-后脑边界、耳泡、内脏弓和体节等多个区域均有表达。在这些区域中，XFGFRL 1/Xndk与XFGF 8共表达，表明XFGFRL 1/Xndk是XFGF 8同表达组的成员，该同表达组包括sprouty、sef和isthmin。(C)2004 Wiley-Liss，Inc.
Fibroblast growth factors (FGFs) mediate many cell-to-cell signaling events during early development. Nou-darake (ndk), a gene encoding an FGF receptor (FGFR)-like molecule, was found to be highly and specifically expressed in the head region of the planarian Dugesia japonica, and its functional analyses provided strong molecular evidence for the existence of a brain-inducing circuit based on the FGF signaling pathway. To analyze the role of ndk during vertebrate development, we isolated the Xenopus ortholog of ndk, the vertebrate FGFR-like 1 gene (XFGFRL1). Expression of XFGFRL1/Xndk was first detected in the anterior region at the late gastrula stage and dramatically increased at the early neurula stage in an overall anterior mesendodermal region, including the prechordal plate, paraxial mesoderm, anterior endoderm, and archenteron roof. This anterior expression pattern resembles that of ndk in planarians, suggesting that the expression of FGFRL1/ndk is conserved in evolution between these two distantly diverged organisms. During the tail bud stages, XFGFRL1/Xndk expression was detected in multiple regions, including the forebrain, eyes, midbrain-hindbrain boundary, otic vesicles, visceral arches, and somites. In many of these regions, XFGFRL1/Xndk was coexpressed with XFGF8, indicating that XFGFRL1/Xndk is a member of the XFGF8 synexpression group, which includes sprouty, sef, and isthmin. (C) 2004 Wiley-Liss, Inc.