Epidemiological surveillance of Campylobacter jejuni in chicken, dairy cattle and diarrhoea patients
Epidemiological surveillance of Campylobacter jejuni in chicken, dairy cattle and diarrhoea patients
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DOI：
10.1017/s0950268809002039
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发表时间：
2009-08-01
影响因子：
4.2
通讯作者：
Jiao, X. A.
中科院分区：
文献类型：
--
作者：
Huang, J. L.;Xu, H. Y.;Jiao, X. A.
应用多重聚合酶链式反应和限制性片段长度多态性技术，从2005年至2006年中国地区鸡、奶牛和腹泻患者中分离到不同亚型的空肠弯曲杆菌。结果表明，在三类样品中，空肠杆菌的检出频率最高，平均分离率为18.61%，鸡群污染率为86.67%。空肠弯曲菌在牛和环境样本、奶牛、小母牛和腹泻患者中的平均发病率分别为7.77%、5.02%、8.70%和4.84%。门诊患者高于病房患者(P<；0.01)，7岁患者高于老年患者(P<；0.01)。对265株空肠弯曲菌的FlaA基因进行了PCR-RFLP分析，将其分为20个不同的类型，其中人的基因分布与家禽和牛的基因分布重叠。这表明，某些空肠弯曲菌菌株在人与牛和家禽等家畜之间传播。
Campylobacter jejuni of different subtypes were identified in chicken, dairy cattle and diarrhoea patients in China from 2005 to 2006, using multiplex PCR and RFLP. The results indicated that, jejuni was most frequently detected in poultry of the three types of the three types of samples, C. jejuni of samples, with an average isolation rate of up to 18.61% and a flock contamination rate of 86.67%. The average incidence of C. jejuni in overall cattle and environmental samples, milk cows, heifers and diarrhoea patients was 7.77, 5.02, 8.70 and 4.84%, respectively. A higher prevalence was detected in outpatients than ward patients (P < 0.01), and in patients aged < 7 years than in older patients (P < 0.01). The 265 isolates of C. jejuni were classified into 20 distinct types by PCR-RFLP analysis of the flaA gene, with the genotype distribution in humans overlapping that in poultry and cattle. This suggests that certain C. jejuni strains circulate between humans and domestic animals such as cattle and poultry.