CO-VARIATION OF NEUROPEPTIDE-Y, CALCITONIN-GENE-RELATED PEPTIDE, SUBSTANCE-P AND NEUROKININ-A IN JOINT FLUID FROM PATIENTS WITH TEMPOROMANDIBULAR-JOINT ARTHRITIS
CO-VARIATION OF NEUROPEPTIDE-Y, CALCITONIN-GENE-RELATED PEPTIDE, SUBSTANCE-P AND NEUROKININ-A IN JOINT FLUID FROM PATIENTS WITH TEMPOROMANDIBULAR-JOINT ARTHRITIS
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DOI：
10.1016/0003-9969(94)00141-w
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发表时间：
1995-02-01
影响因子：
3
通讯作者：
THEODORSSON, E
中科院分区：
文献类型：
--
作者：
ALSTERGREN, P;APPELGREN, A;THEODORSSON, E
41例有颞下颌关节关节炎症状和体征的患者（女性37例，男性4例）分为两个诊断组（I组：炎症性；II组：退行性/非特异性关节疾病）。临床检查，同时用生理盐水抽取关节液，采集静脉血。采用竞争性放射免疫分析法分析关节液和血浆样本的神经肽样免疫反应性，即神经肽Y （NPY-LI）、降钙素基因相关肽（CGRP-LI）、P物质（SP-LI）和神经激肽A （NKA-LI）。目的是研究关节液和血浆中肽的共同变异。I组关节液多肽浓度中值SP-LI = 129, CGRP-LI = 75, NKA-LI = 36, NPY-LI = 676 pmol/l； II组SP-LI = 52, CGRP-LI = 64, NKA-LI = 45, NPY-LI = 318 pmol/l。各组间肽浓度无显著差异。在第一组，所有的神经肽都是强相关的。If组SP-LI和NKA-LI呈强相关，CGRP-LI与NPY-LI和NRA-LI呈弱相关。多元逐步回归分析显示，NPY-LI、CGRP-LI和SP-LI在I组中的大部分变异可以用NKA-LI来解释，但在II组中回归没有达到统计学意义。
Forty-one patients (37 female and four male) with signs and symptoms of temporomandibular joint arthritis, were separated into two diagnostic groups (group I: inflammatory; group II: degenerative/non-specific joint disease). They were examined clinically, fluid was aspirated from the joint with saline and venous blood samples were collected at the same time. The joint fluid and plasma samples were analysed for neuropeptide-like immunoreactivity, i.e. neuropeptide Y (NPY-LI), calcitonin gene-related peptide (CGRP-LI), substance P (SP-LI) and neurokinin A (NKA-LI), using competitive radioimmunoassays. The aim was to investigate any co-variation of the peptides in the joint fluid and plasma. In group I, the median values of peptide concentrations in joint fluid were SP-LI = 129, CGRP-LI = 75, NKA-LI = 36 and NPY-LI = 676 pmol/l and in group II, SP-LI = 52, CGRP-LI = 64, NKA-LI = 45 and NPY-LI = 318 pmol/l. There were no significant differences between the groups for peptide concentrations. In group I, all the neuropeptides were strongly correlated. In group If, SP-LI and NKA-LI were strongly correlated while CGRP-LI was weakly correlated with NPY-LI and NRA-LI. Multiple step-wise regression analysis showed that most of the variation in NPY-LI, CGRP-LI and SP-LI in group I was explained by NKA-LI, but the regression did not reach statistical significance in group II.