The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus.
The control of seed oil polyunsaturate content in the polyploid crop species Brassica napus.
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DOI：
10.1007/s11032-013-9954-5
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Bancroft I
中科院分区：
文献类型：
--
作者：
Wells R;Trick M;Soumpourou E;Clissold L;Morgan C;Werner P;Gibbard C;Clarke M;Jennaway R;Bancroft I
许多重要的植物物种在其近亲祖先中都具有多倍体，这使得对性状变异的遗传基础的推断变得复杂。虽然控制拟南芥种子中多不饱和脂肪酸(PUFAs)比例的主要基因(脂肪酸去饱和酶2；FAD2)是已知的，但另一种相关作物甘蓝型油菜(Brassica Napus)的PUFA含量极低的商业品种尚未开发出来。我们发现，一个油菜品种的多不饱和脂肪酸含量低于正常水平，在四个直源FAD2基因座中的三个上存在非功能等位基因。为了进一步探索遗传基础，我们开发了一个甲烷磺酸乙酯诱变群体JBna CAB_E，并用它来鉴定在剩余功能拷贝中也携带突变的品系。这证实了假设的变异基础，导致了一系列突变株系的等位基因显示出种子油中多不饱和脂肪酸含量的光谱。有几个品系的多不饱和脂肪酸含量为~6%，油酸含量为~84%，实现了长期以来的行业目标：极高油酸，极低多不饱和脂肪酸油菜籽，而不使用转基因技术。该群体含有很高的突变率，是甘蓝型油菜研究的重要资源。本文的在线版本(doi：10.1007/s11032-0139954-5)包含补充材料，可供授权用户使用。
Many important plant species have polyploidy in their recent ancestry, complicating inferences about the genetic basis of trait variation. Although the principal locus controlling the proportion of polyunsaturated fatty acids (PUFAs) in seeds of Arabidopsis thaliana is known (fatty acid desaturase 2; FAD2), commercial cultivars of a related crop, oilseed rape (Brassica napus), with very low PUFA content have yet to be developed. We showed that a cultivar of oilseed rape with lower than usual PUFA content has non-functional alleles at three of the four orthologous FAD2 loci. To explore the genetic basis further, we developed an ethyl methanesulphonate mutagenised population, JBnaCAB_E, and used it to identify lines that also carried mutations in the remaining functional copy. This confirmed the hypothesised basis of variation, resulting in an allelic series of mutant lines showing a spectrum of PUFA contents of seed oil. Several lines had PUFA content of ~6 % and oleic acid content of ~84 %, achieving a long-standing industry objective: very high oleic, very low PUFA rapeseed without the use of genetic modification technology. The population contains a high rate of mutations and represents an important resource for research in B. napus. The online version of this article (doi:10.1007/s11032-013-9954-5) contains supplementary material, which is available to authorized users.
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影响因子：
4.3
作者：
Smith, MA;Moon, H;Kunst, L
通讯作者：
Kunst, L
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11.6
作者：
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作者：
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影响因子：
11.6
作者：
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通讯作者：
Bancroft, Ian
甘蓝型油菜的功能基因组学资源：EMS 诱变群体的开发以及 TILLING 发现的 FAE1 点突变。
影响因子：
9.4
作者：
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通讯作者：
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