Effects of fabrication parameters on viscoelastic shear modulus of 2,3-dialdehydecellulose membranes--potential scaffolds for vocal fold lamina propria tissue engineering.
Effects of fabrication parameters on viscoelastic shear modulus of 2,3-dialdehydecellulose membranes--potential scaffolds for vocal fold lamina propria tissue engineering.
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制造参数对 2,3-二醛纤维素膜粘弹性剪切模量的影响——声带固有层组织工程的潜在支架。
DOI：
10.1002/jbm.a.31921
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Kumar,Vijay
中科院分区：
文献类型：
--
作者：
Roychowdhury,Priyanka;Klemuk,Sarah;Titze,Ingo;Kumar,Vijay
研究了多孔2，3-二异丙基脱纤维素（2，3-DAC）膜用作工程化声带样组织的合成支架。本申请的两个标准是（i）支架的粘弹性剪切性质在声带组织的范围内应该是可控的，和（ii）支架应该保持生物力学稳定以承受生物反应器中的振动应力。多孔2，3-DAC膜由羟甲基纤维素通过水诱导纤维素再生制成，有或没有氯化钠浸出，然后高碘酸盐氧化。它们经过冷冻干燥和环氧乙烷灭菌。在与偏高碘酸钠反应不同时间点获得不同程度的氧化。进行了流变学研究，以研究冷冻干燥、孔隙率、氧化程度、灭菌和孵育时间对弹性和粘性剪切模量G′和G ″的影响，频率分别为0.01-10 Hz。冷冻干燥使G ′和G ″增大，而孔隙率和氧化程度的增大使G ′和G ″减小。灭菌对粘弹性无影响。当在37°C下在Dulbecco最低必需培养基中孵育时，具有6-7%和19-20%氧化的膜分别在7天和3天后崩解，而具有3-4%氧化的膜在42天的时间内显示出很小的粘弹性变化。流变测量的频率上限是本研究的一个局限性，应在未来的研究中加以解决。© 2008 Wiley Periodicals，Inc.生物医学材料研究杂志，2009年
Porous 2,3‐dialdehydecellulose (2,3‐DAC) membranes were investigated for use as a synthetic scaffold for engineering vocal fold‐like tissues. Two criteria of this application are (i) the viscoelastic shear properties of the scaffold should be controllable in the range of vocal fold tissues and (ii) scaffolds should remain biomechanically stable to withstand vibrational stresses in a bioreactor. Porous 2,3‐DAC membranes were fabricated from methylolcellulose by water‐induced cellulose regeneration, with or without sodium chloride leaching, followed by periodate oxidation. They were freeze‐dried and ethylene oxide‐sterilized. Different degrees of oxidation were obtained on reacting with sodium metaperiodate for different time points. Rheological studies were performed to investigate the effect of freeze‐drying, porosity, degree of oxidation, sterilization, and incubation time on elastic and viscous shear moduli,G′ andG″, respectively, for frequencies 0.01–10 Hz. Freeze drying increasedG′ andG″, while increased porosity and degree of oxidation reducedG′ andG″. Sterilization had no effect on viscoelasticity. When incubated in Dulbecco's minimum essential medium at 37°C, membranes with 6–7% and 19–20% oxidation disintegrated after 7 and 3 days, respectively, while membranes with 3–4% oxidation showed little viscoelastic change over a period of 42 days. The upper frequency limit of rheologic measurement was a limitation of the study and should be addressed in future investigations. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009