Prerequisite for the induction of lymphokine-activated killer cells from T lymphocytes.
Prerequisite for the induction of lymphokine-activated killer cells from T lymphocytes.
复制标题
从 T 淋巴细胞诱导淋巴因子激活的杀伤细胞的先决条件。
DOI：
10.4049/jimmunol.140.10.3668
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发表时间：
1988
影响因子：
4.4
通讯作者：
K. Kumagai
中科院分区：
文献类型：
--
作者：
H. Sawada;T. Abo;S. Sugawara;K. Kumagai
通过Percoll梯度法和使用mAb的C介导的细胞溶解的组合，将人血液单核细胞分离成Leu-11+7-NK、Leu-11-7+和Leu-11-7-T细胞。将纯化的Leu-11 - 7+、Leu-11-7+和Leu-11-7-T细胞与rIL-2（500 U/ml）在含10%FCS的培养基中培养6 d，Leu-11+7-NK细胞的增殖反应最强，Leu-11-7+细胞的增殖反应和细胞毒活性中等。另一方面，Leu-11-7-T细胞对rIL-2完全没有应答。然而，当Leu-11-7-T细胞与rIL-2一起在补充有10%自体血清的培养基中培养时，它们显示出对rIL-2相当大的反应性。此外，通过加入单核细胞，产生了对Leu-11-7-T细胞的更大反应。单核细胞细胞因子IL 1、IFN-γ、TNF及其组合均不能替代诱导培养中的单核细胞。相比之下，Leu-11+7- NK细胞的应答水平保持不变，无论向培养基中补充自体血清或向培养物中添加单核细胞。这些结果表明，在实验中的培养条件显着影响的结果，作为测定的淋巴因子激活的杀伤细胞前体，特别是有关的T淋巴细胞转化为淋巴因子激活的杀伤细胞的结果。在适当的条件下，不仅NK细胞，而且T细胞也是淋巴因子激活的杀伤细胞的重要前体。
Human blood mononuclear cells were separated into Leu-11+7-NK, Leu-11-7+, and Leu-11-7-T cells by means of a combination of the Percoll gradient method and C-mediated cytolysis using mAb. When purified Leu-11+7-NK, Leu-11-7+, and Leu-11-7-T cells were cultured with rIL 2 (500 U/ml) for 6 days in a medium supplemented with 10% FCS, Leu-11+7-NK cells responded at the maximum level and Leu-11-7+ cells responded moderately as shown by both cell-proliferation response and cytotoxic activity generated. On the other hand, Leu-11-7-T cells did not respond at all to rIL-2. However, when Leu-11-7-T cells were cultured with rIL-2 in a medium supplemented with 10% autologous serum, they showed considerable responsiveness to rIL-2. In addition, much greater response to Leu-11-7-T cells were produced by the addition of monocytes. Monocyte cytokines, neither IL 1, IFN-gamma, TNF, nor their combination were able to substitute for monocytes in the induction culture. In contrast, the response level of Leu-11+7- NK cells remained unchanged irrespective of supplementation with autologous serum to medium or the addition of monocytes to the culture. These results indicated that culture conditions in the experiments significantly affected the results as to determination of lymphokine-activated killer cell precursors, especially the result pertaining to the conversion of T lymphocytes to lymphokine-activated killer cells. Under appropriate conditions, not only NK cells but also T cells are important precursors of lymphokine-activated killer cells.