Expression profile of doublesex/male abnormal-3-related transcription factor-1 during gonadal sex change in the protogynous wrasse, Halichoeres trimaculatus
Expression profile of doublesex/male abnormal-3-related transcription factor-1 during gonadal sex change in the protogynous wrasse, Halichoeres trimaculatus
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双性/雄性异常 3 相关转录因子 1 在早熟濑鱼 (Halichoeres trimaculatus) 性腺性别变化过程中的表达谱
DOI：
10.1002/mrd.22527
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发表时间：
2015
期刊：
影响因子：
2.5
通讯作者：
Nakamura M
中科院分区：
文献类型：
--
作者：
Nozu R;Horiguchi R;Kobayashi Y;Nakamura M
鱼类的性别变化涉及性腺组织的巨大转变和配子发生的转换。雄性/雄性异常-3相关转录因子-1（DMRT 1）由DMRT 1基因编码，参与多种脊椎动物的睾丸分化以及性分化和性腺性别改变。在本研究中，我们研究了dmrt 1的表达变化在人工性腺性别改变的三点濑鱼，Halichoeres trimaculatus，真实的时间定量PCR和免疫定位，使用我们制备的抗-wrasse-Dmrt 1抗体。结果表明，Dmrt 1主要在睾丸中表达，在睾丸支持细胞和卵巢卵黄发生卵母细胞周围的颗粒细胞中也有表达。此外，dmrt 1表达的上调与生精囊肿数量的增加而不是推测的精原细胞的增殖相一致，这表明dmrt 1参与了性别改变过程中精子发生的进展。性腺性别改变过程中Dmrt 1定位的变化进一步表明，支持细胞起源于三点濑鱼睾丸形成过程中与性腺生殖细胞相邻的体细胞。Reprod. Dev. 82：859-866，2015.© 2015威利期刊公司.
Sex change in fish involves a dramatic transformation of gonadal tissue and a switch in gametogenesis. Doublesex/male abnormal‐3‐related transcription factor‐1 (DMRT1), encoded by theDMRT1gene, is involved in testicular differentiation in a wide range of vertebrates as well as in sexual differentiation and gonadal sex change. In the present study, we investigated changes in the expression ofdmrt1during artificial gonadal sex change in the three‐spot wrasse,Halichoeres trimaculatus, by real‐time quantitative PCR and immunolocalization, using an anti‐wrasse‐Dmrt1 antibody that we prepared. We found thatdmrt1expression was predominantly observed in the testes, and that Dmrt1 was expressed in Sertoli cells of testes and a few granulosa cells surrounding vitellogenic oocytes of the ovary. Additionally, the upregulation ofdmrt1expression was consistent with an increase in spermatogenic cyst quantity rather than proliferation of presumptive spermatogonia, suggesting thatdmrt1is involved in the progression of spermatogenesis during sex change. Changes in the localization of Dmrt1 during gonadal sex change further implied that Sertoli cells originate from somatic cells adjacent to gonial germ cells during testicular formation in the three‐spot wrasse.Mol. Reprod. Dev. 82: 859–866, 2015. © 2015 Wiley Periodicals, Inc.