Do commercial serologic tests for Trypanosoma cruzi infection detect Mexican strains in women and newborns?
Do commercial serologic tests for Trypanosoma cruzi infection detect Mexican strains in women and newborns?
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DOI：
10.1645/ge-2545.1
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
Dumonteil E
中科院分区：
文献类型：
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作者：
Gamboa-León R;Gonzalez-Ramirez C;Padilla-Raygoza N;Sosa-Estani S;Caamal-Kantun A;Buekens P;Dumonteil E
我们试图确定可用于墨西哥克氏锥虫血清阳性率研究的血清学测试，在墨西哥，血统I占主导地位。在之前对尤卡坦州和瓜纳华托州孕妇及其新生儿进行的一项研究中，我们报告了Stat-Pak和Wiener ELISA法检测克氏锥虫特异性抗体的0.8-0.9%的患病率。我们在这里扩展了这项研究，进行了另一种非商业性的ELISA法，并使用当地寄生虫株的完整抗原，用Western印迹法确认了血清阳性。我们发现梅里达和瓜纳华托的血清阳性率分别为0.6%(3/500)和0.4%(2/488)。5份血清阳性的脐带标本对非商业性ELISA法和免疫印迹试验均呈阳性反应，只有1份母体标本对非商业性ELISA法不反应。在尤卡坦对新生儿进行了10个月的随访，以确定儿童中是否存在克鲁兹旋毛虫抗体作为先天性感染的证据。没有一个孩子的血清呈阳性。一名感染母亲的新生儿在心脏骤停2周时死亡，但克氏支原体感染尚未得到证实。用两种商业检测(Stat-Pak和EL ISA Wiener)获得的克鲁兹毛滴虫血清阳性率数据与使用墨西哥当地的一种毛滴虫毒株进行的非商业测试得到的数据相似。
We sought to determine the serological test that could be used for Trypanosoma cruzi seroprevalence studies in Mexico, where lineage I predominates. In a previous study among pregnant women and their newborns in the states of Yucatan and Guanajuato, we reported a 0.8–0.9% of prevalence for T. cruzi–specific antibodies by Stat-Pak and Wiener ELISA. We have expanded this study here by performing an additional non-commercial ELISA and confirming the seropositives with Western blot, using whole antigens of a local parasite strain. We found a seroprevalence of 0.6% (3/500) in Merida and 0.4% in Guanajuato (2/488). The 5 seropositive umbilical cord samples reacted to both non-commercial ELISA and Western blot tests, and only 1 of the maternal samples was not reactive to non-commercial ELISA. A follow-up of the newborns at 10 mo was performed in Yucatan to determine the presence of T. cruzi antibodies in children as evidence of congenital infection. None of the children was seropositive. One newborn from an infected mother died at 2 wk of age of cardiac arrest, but T. cruzi infection was not confirmed. The T. cruzi seroprevalence data obtained with both commercial tests (Stat-Pak and ELISA Wiener) are similar to those from non-commercial tests using a local Mexican strain of T. cruzi.