Extrusion 3D-printed tricalcium phosphate-polycaprolactone biocomposites for quercetin-KCl delivery in bone tissue engineering.
Extrusion 3D-printed tricalcium phosphate-polycaprolactone biocomposites for quercetin-KCl delivery in bone tissue engineering.
复制标题
挤出 3D 打印磷酸三钙-聚己内酯生物复合材料，用于骨组织工程中槲皮素-KCl 的输送。
DOI：
10.1002/jbm.a.37692
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Bose,Susmita
中科院分区：
文献类型：
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作者：
Toulou,Connor;Chaudhari,VishalSharad;Bose,Susmita
由于有限的骨组织再生能力，临界大小的骨缺损在先进的医疗保健中构成了巨大的挑战。众多重叠变量的复杂相互作用阻碍了多功能生物复合材料的发展。植物化学物质在促进骨骼生长方面显示出很好的前景，但它们的剂量依赖性和物理化学性质阻碍了临床应用。为了开发一种全面的解决方案，在三维打印(3DP)挤出磷酸三钙-聚己内酯(TCP-PCL)支架上添加了槲皮素和氯化钾(KCl)。这种复合材料的抗压强度为30 Mpa，在低负荷应用中表现出良好的稳定性。从支架中释放的槲皮素遵循两相模式，通过扩散介导的动力学驱动，可持续长达28 天。KCl的加入允许支架的降解速率可调，并防止最初的快速释放。支架的功能化促进了人胎儿成骨细胞(HfOB)的附着和增殖，使细胞活力提高了2.1倍。与未经处理的基质相比，经处理的支架显示骨肉瘤(MG-63)细胞存活率降低3倍。细胞形态的破裂和线粒体膜电位的降低表明其具有抗肿瘤的潜能。负载了槲皮素和槲皮素氯化钾(Q-KCl)的支架对金黄色葡萄球菌的菌落数分别减少了76%和89%。本研究为骨组织工程(BTE)在骨科修复中的应用提供了一种有前景的策略。
Critical‐sized bone defects pose a significant challenge in advanced healthcare due to limited bone tissue regenerative capacity. The complex interplay of numerous overlapping variables hinders the development of multifunctional biocomposites. Phytochemicals show promise in promoting bone growth, but their dose‐dependent nature and physicochemical properties halt clinical use. To develop a comprehensive solution, a 3D‐printed (3DP) extrusion‐based tricalcium phosphate‐polycaprolactone (TCP‐PCL) scaffold is augmented with quercetin and potassium chloride (KCl). This composite material demonstrates a compressive strength of 30 MPa showing promising stability for low load‐bearing applications. Quercetin release from the scaffold follows a biphasic pattern that persists for up to 28 days, driven via diffusion‐mediated kinetics. The incorporation of KCl allows for tunable degradation rates of scaffolds and prevents the initial rapid release. Functionalization of scaffolds facilitates the attachment and proliferation of human fetal osteoblasts (hfOB), resulting in a 2.1‐fold increase in cell viability. Treated scaffolds exhibit a 3‐fold reduction in osteosarcoma (MG‐63) cell viability as compared to untreated substrates. Ruptured cell morphology and decreased mitochondrial membrane potential indicate the antitumorigenic potential. Scaffolds loaded with quercetin and quercetin‐KCl (Q‐KCl) demonstrate 76% and 89% reduction in bacterial colonies ofStaphylococcus aureus, respectively. This study provides valuable insights as a promising strategy for bone tissue engineering (BTE) in orthopedic repair.