Processing and nucleo-cytoplasmic transport of histone gene transcripts.
Processing and nucleo-cytoplasmic transport of histone gene transcripts.
复制标题
组蛋白基因转录物的加工和核质转运。
DOI：
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发表时间：
1984
影响因子：
14.9
通讯作者：
M. Birnstiel
中科院分区：
文献类型：
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作者：
O. Georgiev;J. Mous;M. Birnstiel
用SP6转录系统体外合成非洲爪蟾和海胆组蛋白mRNA的前体，并通过蛙卵母细胞注射研究其成熟和核质转运。同源组蛋白信使序列的3'加工最快，并且不需要前体的真正5'或特异性3'末端，但是5'末端的加帽强烈影响3'加工的效率。当将含有5'间隔区序列延伸的前体注射到卵母细胞核中时，不能检测到5'组蛋白mRNA末端的产生。这一发现可能对组蛋白基因转录是否为多顺反子的问题有一定的启示。使用一种新的卵母细胞技术，我们已经从细胞质中分离出细胞核，并研究了处理后的RNA从卵母细胞核进入细胞质的时间过程。结果表明，RNA成熟和核质运输不是时间耦合的过程。
Precursors of Xenopus and sea urchin histone mRNAs were synthesized in vitro with the SP6 transcription system, and their maturation and nucleo-cytoplasmic transport was studied by frog oocyte injection. 3' processing is most rapid for homologous histone messenger sequences and does not require either genuine 5' or specific 3' ends of the precursor, but capping of the 5' terminus strongly influences the efficiency of 3' processing. No generation of 5' histone mRNA ends can be detected when precursors containing 5' spacer sequence extensions are injected into the oocyte nucleus. This finding may have some implications for the question whether histone gene transcription could be polycistronic. Using a novel oocyte technique, we have separated nuclei from cytoplasm and have studied the time course of exit of the processed RNA from the oocyte nucleus into the cytoplasm. The results suggest that RNA maturation and nucleo-cytoplasmic transport are not temporally coupled processes.