Polymorphism analysis of seven Y-STR loci in Shui population in Guizhou, China
Polymorphism analysis of seven Y-STR loci in Shui population in Guizhou, China
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DOI：
10.1360/yc-006-1495
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发表时间：
2006-12-01
期刊：
影响因子：
--
通讯作者：
Ren Xi-Lin
中科院分区：
文献类型：
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作者：
He Yan;Shan Ke-Ren;Ren Xi-Lin
本研究的目的是调查中国贵州水族人群中7个Y特异性STR位点的多态性，以获得该少数民族的多态性信息。用荧光标记引物同时扩增1个三核苷酸STR基因座和6个四核STIR基因座，并用ABI PRISM (TM) 377 DNA测序仪测定基因型。计算等位基因频率、遗传多样性和单倍型多样性。在 94 名无关男性中，在 DYS19、DYS389 I、DYS38911、DYS390、DYS391、DYS392 和 DYS393 位点分别观察到 6、4、6、2、3、5、4 个等位基因。总共，7 个 Y-STR 位点鉴定出了 27 个单倍型。每个位点的遗传多样性值范围为 0.124 (DYS389 I) 至 0.630 (DYS19)。单倍型多样性值为0.868。贵州水族人群存在较高的单倍型多样性。研究表明，这七个 Y-STR 位点是有价值的 Y 特异性标记，可用于建立 Y-STR 数据库、了解种族起源和迁徙以及个人识别。
The objective of this study was to investigate the polymorphism of seven Y-specific STR loci in Shui ethnic population of Guizhou, China, and to obtain the polymorphism information in this minority. One trinucleotide STR locus and six tetranucleoticle STIR loci were simultaneously amplified with fluorescently labeled primers, and genotypes were determined with ABI PRISM (TM) 377 DNA Sequencer. Allele frequencies, genetic diversity and haplotype diversity were calculated. Among 94 unrelated males, 6, 4, 6, 2, 3, 5, 4 alleles were observed in loci DYS19, DYS389 I, DYS38911, DYS390, DYS391, DYS392 and DYS393, respectively. Altogether, 27 haplotypes were identified for the seven Y-STR loci. The genetic diversity values for each locus ranged from 0.124 (DYS389 I) to 0.630 (DYS19). The haplotype diversity value was 0.868. High haplotype diversities were found in Shui population of Guizhou. The study suggests that these seven Y-STR loci are valuable Y-specific markers for establishing a Y-STR database, understanding ethical origin and migrations and for personal identification.