Responses of seedling growth and antioxidant activity to excess iron and copper in Triticum aestivum L.
Responses of seedling growth and antioxidant activity to excess iron and copper in Triticum aestivum L.
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小麦幼苗生长和抗氧化活性对过量铁和铜的响应。
DOI：
10.1016/j.ecoenv.2012.09.010
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发表时间：
2012-12
影响因子：
6.8
通讯作者：
Haijian Chen
中科院分区：
文献类型：
--
作者：
Lingyun Jia;Tengguo Zhang;Yingli Yang;Haijian Chen
本研究旨在分析短期铁(Fe)和铜(Cu)胁迫(0、100、300和500μM)对小麦幼苗根系生长的毒害机制，并比较其叶片的生理变化。不同浓度的铁和铜处理均能缩短幼苗根和地上部的长度，但只有在500μM时铜才能抑制种子萌发。荧光染料2‘，7’-二氯二氢荧光素二乙酸酯分析表明，铁和铜处理提高了幼苗根中的过氧化氢水平。与铁处理相比，相同浓度的铜胁迫导致根部细胞活力显著降低，膜脂损伤严重。叶片总叶绿素含量(包括Chla和Chlb)随着Fe浓度的升高而显著增加，而最高浓度(500μM)则导致包括Chla在内的总Chl含量显著下降。此外，Fe胁迫刺激叶片过氧化物酶和抗坏血酸过氧化物酶活性，但最高铁浓度对叶片抗坏血酸过氧化物酶活性有抑制作用。相反，铜处理提高了叶片过氧化氢酶和过氧化物酶活性。因此，在相同浓度下，铜处理的叶片中过氧化氢含量显著低于铁处理。
The purpose of this study was to analyze phytotoxicity mechanism involved in root growth and to compare physiological changes in the leaves of wheat seedlings exposed to short term iron (Fe) and copper (Cu) stresses (0, 100, 300 and 500μM). All applied Fe or Cu concentrations reduced root and shoot lengths, but seed germination was inhibited by Cu only at 500μM. Analyses using fluorescent dye 2′,7′-dichlorodihydrofluorescein diacetate indicated enhanced H2O2levels in seedling roots under Fe and Cu treatments. Cu stress at the same concentration induced a great reduction in cell viability and a strong damage on membrane lipid in the roots with respect to Fe treatment. Significant increases in the total chlorophyll (chl) content including chl a and chl b were observed in response to higher Fe concentrations, whereas the highest Cu concentration (500μM) led to significant decreases in the total chl content including chl a. Additionally, leaf peroxidase (POD) and ascorbate peroxidase (APX) were stimulated by Fe stress, but the highest Fe concentration exhibited inhibitory effect on leaf APX activity. In contrast, copper treatment resulted in an elevation in leaf catalase and POD activities. Therefore, H2O2content in the leaves associated with copper was significantly lower than that with iron at the same concentration.
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影响因子：
1.8
作者：
Philippa Nichols;James D Couch;S. Al-Hamdani
通讯作者：
Philippa Nichols;James D Couch;S. Al-Hamdani
影响因子：
4.9
作者：
Y. Nakano;K. Asada
通讯作者：
Y. Nakano;K. Asada
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
V. Nenova;M. Popov
通讯作者：
V. Nenova;M. Popov
影响因子：
6.8
作者：
Bouazizi, Houda;Jouili, Hager;El Ferjani, Ezzeddine
通讯作者：
El Ferjani, Ezzeddine
影响因子：
6.8
作者：
Posmyk, M. M.;Kontek, R.;Janas, K. M.
通讯作者：
Janas, K. M.