The suppressing effects of BTG3 expression on aggressive behaviors and phenotypes of colorectal cancer: An in vitro and vivo study.
The suppressing effects of BTG3 expression on aggressive behaviors and phenotypes of colorectal cancer: An in vitro and vivo study.
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BTG3表达对结直肠癌攻击行为和表型的抑制作用：体外和体内研究
DOI：
10.18632/oncotarget.15438
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发表时间：
2017-03-14
期刊：
影响因子：
--
通讯作者：
Li ZJ
中科院分区：
文献类型：
--
作者：
Zheng HC;He HY;Wu JC;Li J;Zhao S;Zhao GF;Jiang HM;Yu XW;Li ZJ
在此，我们发现BTG 3的表达下调可能与结直肠癌的发生呈正相关，并且其过表达抑制SW 480和SW 620细胞的增殖、糖酵解、线粒体呼吸、细胞周期进程、迁移和侵袭，并诱导凋亡、衰老和分化。经顺铂、MG 132、紫杉醇和SAHA处理后，BTG 3转染细胞的存活率低于对照组，凋亡率高于对照组，且呈时间和剂量依赖性。BTG 3过表达可上调SW 480和SW 620细胞Cyclin E、p16、p27、NF-κB、p38α/β、XIAP、Bcl-2、ATG 14和p53蛋白表达，下调MRP 1、BCRP和mTOR mRNA表达。BTG 3过表达通过抑制细胞增殖和诱导细胞凋亡抑制肿瘤生长。提示BTG 3表达下调可能是结直肠癌发生的一个标志。BTG 3过表达可能逆转大肠癌的侵袭性表型，并可能成为大肠癌基因治疗的潜在靶点。
Here, we found that down-regulated expression of BTG3 might be positively correlated with colorectal carcinogenesis and its overexpression suppressed proliferation, glycolysis, mitochondrial respiration, cell cycle progression, migration, and invasion, and induced apoptosis, senescence and differentiation in SW480 and SW620 cells. After treated with cisplatin, MG132, paclitaxel and SAHA, BTG3 transfectants exhibited lower viability and higher apoptosis than the control in both time- and dose-dependent manners. BTG3 overexpression up- regulated the protein expression of Cyclin E, p16, p27, NF-κB, p38α/β, XIAP, Bcl-2, ATG14 and p53, but down-regulated the mRNA expression of MRP1, BCRP, and mTOR in SW480 and SW620 cells. BTG3 overexpression inhibited tumor growth of SW620 cells by suppressing proliferation and inducing apoptosis. It was suggested that down-regulated BTG3 expression might be considered as a marker for colorectal carcinogenesis. BTG3 overexpression might reverse the aggressive phenotypes and be employed as a potential target for gene therapy of colorectal cancer.