Spontaneous propagating calcium waves underpin airway peristalsis in embryonic rat lung
Spontaneous propagating calcium waves underpin airway peristalsis in embryonic rat lung
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DOI：
10.1165/rcmb.2005-0137oc
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发表时间：
2005-08-01
影响因子：
6.4
通讯作者：
Burdyga, TV
中科院分区：
文献类型：
--
作者：
Featherstone, NC;Jesudason, EC;Burdyga, TV
来自不同物种的产前气道表现出自发的蠕动收缩（气道蠕动）。这些收缩波似乎与产前肺生长有关，并可能起调节作用。它们不受阿托品或河豚毒素的影响，但被硝苯地平消除。然而，这些收缩波产生、调节和传播的机制仍然不清楚。利用钙成像和全胚胎肺器官培养，我们首次证明了胚胎气道的肠功能是由自发的、可再生的、温度敏感的钙（Ca2+）波驱动的。这些Ca2+波通过间隙连接在单个气道平滑肌细胞之间传播，可能是动作电位介导的，不仅依赖于细胞外钙通过l型电压门控通道进入，还依赖于细胞内Ca2+储存。因此，如果气道蠕动调节肺生长，这些发现意味着气道平滑肌Ca2+波反过来调节产前肺形态发生。
Prenatal airways from diverse species exhibit spontaneous peristaltic contractions (airway peristalsis). These contractile waves appear coupled to and may function to regulate prenatal lung growth. They are unaffected by atropine or tetrodotoxin but abolished by nifedipine. Nevertheless, the mechanisms by which these contractile waves are generated, regulated, and propagated remain obscure. Using calcium imaging and whole embryonic lung organ culture, we demonstrate for the first time that peristalsis of the embryonic airway is driven by spontaneous, regenerative, temperature-sensitive calcium (Ca2+) waves. These Ca2+ waves propagate between individual airway smooth muscle cells coupled via gap junctions, are likely to be action potential-mediated, and are dependent on not only extracellular calcium entry via L-type voltage-gated channels but also intracellular Ca2+ stores. Thus, if airway peristalsis regulates lung growth, these findings mean that airway smooth muscle Ca2+ waves in turn regulate prenatal lung morphogenesis.