Ultrafast identification of Pinelliae Rhizoma using colorimetric direct-VPCR
Ultrafast identification of Pinelliae Rhizoma using colorimetric direct-VPCR
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DOI：
10.1007/s13205-021-03035-9
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发表时间：
2021-11
期刊：
影响因子：
2.8
通讯作者：
Rongqian Chen;Sheng Ding;Yinghua Wei;Jia-wen Yu;Rui-yu Xu;Xiao Luo;Gang Fan;Hongxiang Yin;Jinhui Bian
中科院分区：
文献类型：
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作者：
Rongqian Chen;Sheng Ding;Yinghua Wei;Jia-wen Yu;Rui-yu Xu;Xiao Luo;Gang Fan;Hongxiang Yin;Jinhui Bian
随着中草药市场的日益增长，对于海量中草药样品的高通量鉴定，迫切需要一种快速、易用的系统。在此基础上，设计了一种基于简化模板制备和一种新的扩增技术的超快速比色检测系统，称为比色直接VPCR。该系统已成功应用于半夏药材的鉴别。与传统方法相比，从样品处理到检测结果，整个测试可以在30分钟内完成。通过对来自9个不同生境的72个样品的正确识别，对该方法进行了评估，表明该方法具有较高的可靠性。综上所述，我们提出了一种基于DIRECT-VPCR的超高速(小于30min)和比色检测平台(紫外灯下)用于半夏的鉴定。该流水线具有较高的实用性(100%准确率)，在其他中草药的常规检测中具有良好的应用前景。
With the increasing growth of the herbal market, a rapid and easy-to-use system is highly desirable in the high-throughput identification of massive herbal medicine samples. Here, an ultrafast and colorimetric detection system was devised based on simplifying template preparation and a newly developed amplification technique, named colorimetric direct-VPCR. The system was successfully applied to the identification of Pinelliae Rhizoma. Compared to the traditional method, the whole test can be finished within 30 min from the sample treatment to the testing results. The method was evaluated by correctly identifying 72 samples obtained from 9 different habitats, demonstrating its high reliability. In summary, we present an ultrafast (less than 30 min) and colorimetric detection platform (under ultraviolet lamp) based on direct-VPCR for the identification of Pinelliae Rhizoma. The high practicability (100% accuracy) of this pipeline enables it to be a promising method in the routine detection of other herbal materials.