Orthotopic transplantation of LH receptor knockout and wild-type ovaries.
Orthotopic transplantation of LH receptor knockout and wild-type ovaries.
复制标题
LH 受体敲除和野生型卵巢的原位移植。
DOI：
10.1016/j.lfs.2005.03.024
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Rao,ChV
中科院分区：
文献类型：
--
作者：
Chudgar,Daksha;Lei,Zhenmin;Rao,ChV
促黄体生成激素（LH）受体敲除动物由于窦期卵泡发生停滞而导致卵巢功能衰竭。结果，动物具有不育表型。本研究旨在确定这种表型是否可以通过野生型卵巢原位移植逆转。结果表明，将野生型卵巢移植到无效动物中不会导致发情周期的恢复。虽然不同类型的卵泡数量增加，但没有进展到排卵。血清激素水平改善，反映了卵巢的变化。卵巢缺失的野生型动物也无法进行周期，它们的卵巢和血清激素水平更像是卵巢缺失的动物。尽管预测了置于野生型动物中的无效卵巢缺乏拯救，但置于无效动物中的野生型卵巢失败，这可能是由于移植组织长期暴露于高循环LH水平，也可能是由于无效动物的内部环境改变。这些发现可能对将来考虑将正常供体卵巢移植到因LH受体失活突变导致卵巢衰竭的妇女中具有潜在的意义。
Luteinizing hormone (LH) receptor knockout animals have an ovarian failure due to an arrest in folliculogenesis at the antral stage. As a result, the animals have an infertility phenotype. The present study was undertaken to determine whether this phenotype could be reversed by orthotopic transplantation of wild-type ovaries. The results revealed that transplanting wild-type ovaries into null animals did not result in resumption of estrus cycles. Although the number of different types of follicles increased, none progressed to ovulation. The serum hormone profiles improved, reflecting the ovarian changes. The wild-type animals with null ovaries also failed to cycle and their ovaries and serum hormone levels were more like null animals with their own ovaries. Although the lack of rescue of null ovaries placed into wild-type animals was predicted, the failure of wild-type ovaries placed in null animals was not, which could be due to chronic exposure of transplanted tissue to high circulating LH levels and also possibly due to altered internal milieu in null animals. These findings may have implications for potential future considerations of grafting normal donor ovaries into women who have an ovarian failure resulting from inactivating LH receptor mutations.