ANTIGEN RECOGNITION IN AUTOIMMUNE ENCEPHALOMYELITIS AND THE POTENTIAL FOR PEPTIDE-MEDIATED IMMUNOTHERAPY
ANTIGEN RECOGNITION IN AUTOIMMUNE ENCEPHALOMYELITIS AND THE POTENTIAL FOR PEPTIDE-MEDIATED IMMUNOTHERAPY
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DOI：
10.1016/0092-8674(89)90287-0
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发表时间：
1989-10-20
期刊：
影响因子：
64.5
通讯作者：
MCDEVITT, HO
中科院分区：
文献类型：
--
作者：
WRAITH, DC;SMILEK, DE;MCDEVITT, HO
多肽结合和淋巴T细胞活化研究已经被用来表征T细胞对来自髓鞘碱性蛋白的脑源性T细胞自身抗原的识别。SJL)F1小鼠。定义了决定与主要组织相容性复合体(MHC)的限制元件或脑源性T细胞受体相互作用的氨基酸。这一信息使设计与MHC结合但不与自身抗原发生交叉反应的多肽成为可能。体外实验表明，生脑T细胞表位的多肽类似物对MHC的结合和激活具有“异质性”作用。这种类似物对体内的脑源性T细胞不具有免疫原性，并被证明可以抑制由自身抗原本身诱导的疾病。
Peptide binding and lymph node T cell activation studies have been used to characterize T cell recognition of an encephalitogenic T cell autoantigen from myelinbasic protein in (PL/J .times. SJL)F1 mice. Amino acids that determine interactions with either the restriction element of the major histocompatibility complex (MHC) or the encephalitogenic T cell receptor are defined. This information enables the design of peptides that bind MHC yet do not cross-react with the autoantigen. A peptide analog of the encephalitogenic epitope is shown to be "heteroclitic" for MHC binding and activation of encephalitogenic T cells in vitro. This analog is not immunogenic for encephalitogenic T cells in vivo and is shown to inhibit disease that is induced by the autoantigen itself.