Changes in the SDS elutability of fibrinogen adsorbed from plasma to polymers.
Changes in the SDS elutability of fibrinogen adsorbed from plasma to polymers.
复制标题
从血浆吸附到聚合物的纤维蛋白原的 SDS 洗脱能力的变化。
DOI：
10.1163/156856289x00091
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Horbett,TA
中科院分区：
文献类型：
--
作者：
Rapoza,RJ;Horbett,TA
将狒狒纤维蛋白原从稀释的血浆溶液中吸附到玻璃、聚乙烯、聚苯乙烯、聚二甲基硅氧烷、聚（乙烯）对苯二甲酸酯和Biomer®。吸附后，一半的样品立即放入十二烷基硫酸钠（SDS）溶液中洗脱蛋白质，其余样品在缓冲液中保存5天，然后在SDS中洗脱。洗脱通常不完全，但取决于血浆浓度和停留时间。从更稀释的血浆中吸附的纤维蛋白原的洗脱率通常较低，对于纤维蛋白原在其上停留5天的样品，洗脱率明显较低。蛋白质层的不可洗脱部分在聚苯乙烯上迅速形成，而在聚乙烯上最初的洗脱率很高，随后逐渐降低。
Baboon fibrinogen was adsorbed from diluted plasma solutions to glass, polyethylene, polystyrene, polydimethylsiloxane, poly(ethylene)terephthalate, and Biomer®. Following adsorption, half of the samples were immediately placed in sodium dodecyl sulfate (SDS) solutions to elute the protein, while the others were stored in buffer for up to 5 days and then eluted in SDS. The elution was typically incomplete, but depended on the plasma concentration and the residence time. The elutability was generally lower for fibrinogen adsorbed from more diluted plasma, and substantially lower for samples on which the fibrinogen had resided for 5 days. The non-elutable portion of the protein layer formed rapidly on polystyrene, while on polyethylene elutability was high initially, followed by a gradual decrease.