Controllable shrinking of glass capillary nanopores down to sub-10 nm by wet-chemical silanization for signal-enhanced DNA translocation
Controllable shrinking of glass capillary nanopores down to sub-10 nm by wet-chemical silanization for signal-enhanced DNA translocation
复制标题
通过湿化学硅烷化将玻璃毛细管纳米孔可控收缩至 10 nm 以下，以实现信号增强的 DNA 易位
DOI：
10.1021/acssensors.7b00385
复制
发表时间：
2017
期刊：
影响因子：
8.9
通讯作者：
Jin Yongdong
中科院分区：
文献类型：
--
作者：
Xu Xiaolong;Li Chuanping;Zhou Ya;Jin Yongdong
直径是纳米孔传感的主要关注点。然而，直接拉取直径小于10纳米的玻璃毛细管纳米孔是非常困难的。因此，后期处理有时是必要的。在此，我们展示了一种简单有效的湿化学方法，通过硅酸二钠水解将玻璃毛细管纳米孔的直径从几十纳米缩小到10纳米以下。研究了其对DNA易位的益处。玻璃毛细管纳米孔的缩小不仅减缓了DNA的易位，而且显著提高了DNA易位信号和信噪比（6.4 nm玻璃纳米孔为102.9，优于3 nm氮化硅纳米孔为15）。它还会影响DNA易位行为，使得该方法和玻璃毛细管纳米孔平台有望用于DNA易位研究。
Diameter is a major concern for nanopore based sensing. However, directly pulling glass capillary nanopore with diameter down to sub-10 nm is very difficult. So, post treatment is sometimes necessary. Herein, we demonstrate a facile and effective wet-chemical method to shrink the diameter of glass capillary nanopore from several tens of nanometers to sub-10 nm by disodium silicate hydrolysis. Its benefits for DNA translocation are investigated. The shrinking of glass capillary nanopore not only slows down DNA translocation, but also enhances DNA translocation signal and signal-to-noise ratio significantly (102.9 for 6.4 nm glass nanopore, superior than 15 for a 3 nm silicon nitride nanopore). It also affects DNA translocation behaviors, making the approach and glass capillary nanopore platform promising for DNA translocation studies.