Identification of Limulus polyphemus haemocyanin messenger RNA.
Identification of Limulus polyphemus haemocyanin messenger RNA.
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鲎血蓝蛋白信使RNA的鉴定。
DOI：
10.1042/bj1960653
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Bonaventura,J
中科院分区：
文献类型：
--
作者：
Wood,EJ;Bonaventura,J
总RNA分离自含成氰细胞的组织，取自鲎（Limulus polyphemus）复眼后面。在存在L-[35 S]甲硫氨酸的情况下，在兔网织红细胞溶血物系统中翻译通过在寡聚（dT）-纤维素上的亲和层析从中分离的含聚（A）的RNA。通过使用鲎血蓝蛋白的抗血清，从翻译产物中分离出多肽，其具有与真实鲎血蓝蛋白多肽相似的迁移率，如通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳所判断的。
Total RNA was isolated from cyanoblast-containing tissue taken from behind the compound eye of the horseshoe crab, Limulus polyphemus. Poly(A)-containing RNA separated from this by affinity chromatography on oligo(dT)-cellulose was translated in the rabbit reticulocyte haemolysate system in the presence of L-[35S]methionine. By using an antiserum to Limulus haemocyanin, polypeptides were isolated from the translation products which had a similar mobility to the authentic Limulus haemocyanin polypeptides as judged by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis.