Modulation of the Induction of Lung and Airway Allergy in the Offspring of IFN-γ-Treated Mother Mice1
Modulation of the Induction of Lung and Airway Allergy in the Offspring of IFN-γ-Treated Mother Mice1
复制标题
IFN-γ 治疗的母鼠后代肺和气道过敏的诱导调节1
作者：
C. Lima;V. Souza;E. Faquim;M. Hoshida;E. Bevilacqua;M. Macedo;W. Tavares;B. Vargaftig
最近的研究强调了胎儿/母亲的相互作用对哮喘发展的影响。因为干扰素-γ减少了Th2介导的过敏反应，所以我们评估了在怀孕期间注射给母亲时，它对后代哮喘的调节能力。于妊娠第6.5天给CD1雌性小鼠注射干扰素-γ。出生后，雄性新生儿立即被关在笼子里与母亲互换：干扰素-γ治疗的母亲的后代由正常母亲哺乳(干扰素/NOR)，正常母亲的婴儿由干扰素-γ治疗的母亲(NOR/NOR)或正常母亲(NOR/NOR)哺乳。在ConA刺激下，断奶后即刻，NOR和NOR/NOR小鼠的脾细胞产生的IL-4比NOR/NOR小鼠低，而产生的干扰素-γ比NOR/NOR小鼠高。NOR/NOR小鼠的支气管肺泡灌洗液显示嗜酸性粒细胞，大量嗜酸性细胞存在于肺组织的血管周围和支气管区。两组小鼠肺泡灌洗液中嗜酸性粒细胞数量均显著减少。此外，干扰素/NOR小鼠的肺切片显示几乎正常的组织学，但不是NOR/干扰素小鼠。与OVA致敏的NOR/NOR组相比，OVA致敏的NOR和NOR组小鼠脾细胞产生的干扰素-γ、IL-4和IL-5显著减少。干扰素/NOR组小鼠血浆中的IgE和过敏性IgG1也明显降低。总之，妊娠期间干扰素-γ的存在为胎儿提供了一种保护，使其在成年后免受过敏性刺激。
Recent studies have highlighted the influence of fetal/maternal interactions on the development of asthma. Because IFN-γ reduces Th2-mediated allergic responses, we assessed its capacity to modulate asthma in the offspring when injected into mothers during pregnancy. IFN-γ was injected in CD1 female mice on day 6.5 of gestation. Immediately after birth, male newborns were housed in cages with interchanged mothers: the offspring from IFN-γ-treated mothers were breastfed by normal mothers (IFN/nor), and those from normal mothers were breastfed by IFN-γ-treated (Nor/IFN) or normal mothers (Nor/nor). Immediately after weaning, the spleen cells from IFN/nor and Nor/IFN mice produced less IL-4 and more IFN-γ than Nor/nor mice when stimulated with Con A. At the age of 6–7 wk, mice were immunized with OVA on days 0 and 7. From day 14 to 16, they were exposed to aerosolized OVA. The bronchoalveolar lavage fluid from Nor/nor mice showed eosinophilia, a large number of these cells being present in perivascular and peribronchial regions of lung tissues. IFN/nor or Nor/IFN mice showed greatly reduced eosinophil numbers in bronchoalveolar lavage fluid. In addition, lung sections from IFN/nor, but not Nor/IFN mice showed almost normal histology. In OVA-sensitized IFN/nor and Nor/IFN mice, the production of IFN-γ, IL-4, and IL-5 by spleen cells was significantly reduced as compared with cells from the OVA-sensitized Nor/nor group. IgE and anaphylactic IgG1 were also reduced in plasma of IFN/nor mice. In conclusion, the presence of IFN-γ during pregnancy confers to the fetus a protection against allergenic provocations in the adult life.
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促肾上腺皮质激素释放因子和白介素-1 在控制生殖功能的神经元调节中的作用。
DOI：
10.1210/edrv-16-2-177
发表时间：
1995
期刊：
Endocrine reviews.
影响因子：
--
作者：
Rivest,S;Rivier,C
通讯作者：
Rivier,C
DOI：
10.1002/9780470514849.ch14
发表时间：
1995
期刊：
Ciba Foundation symposium
影响因子：
--
作者：
G. Trinchieri
通讯作者：
G. Trinchieri
粘膜 IFN-γ 基因转移抑制小鼠肺部过敏反应。
DOI：
--
发表时间：
1996
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Li,XM;Chopra,RK;Chou,TY;Schofield,BH;Wills-Karp,M;Huang,SK
通讯作者：
Huang,SK
影响因子：
4.4
作者：
R. Schleimer;S. A. Sterbinsky;James Kaiser;C. Bickel;David Klunk;K. Tomioka;W. Newman;F. Luscinskas;M. Gimbrone;B. McIntyre
通讯作者：
R. Schleimer;S. A. Sterbinsky;James Kaiser;C. Bickel;David Klunk;K. Tomioka;W. Newman;F. Luscinskas;M. Gimbrone;B. McIntyre