Effects of brewing conditions on the antioxidant capacity of twenty-four commercial green tea varieties.
Effects of brewing conditions on the antioxidant capacity of twenty-four commercial green tea varieties.
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DOI：
10.1016/j.foodchem.2015.07.005
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发表时间：
2016-02-01
期刊：
影响因子：
8.8
通讯作者：
Bradley R
中科院分区：
文献类型：
--
作者：
Sharpe E;Hua F;Schuckers S;Andreescu S;Bradley R
一种新的纸基纳米氧化铈还原抗氧化能力（NanoCerac）测定抗氧化剂检测，已被改编为第一次作为一个高通量的方法，以测量酿造条件和再灌注对24个商业绿色茶的抗氧化能力的影响。氧自由基吸收能力（ORAC）测定，经常应用于复杂的食品和饮料，被用作抗氧化能力的比较措施。持续抗氧化能力的一种新测量方法，总固有抗氧化能力（TI-NanoCerac和TI-ORAC）通过将每种茶浸泡六次来测量。酿造条件（温度、酿造时间等）的影响以一种受欢迎的茶为标准进行评估。NanoCerac和ORAC测定均中度相关（R2 0.80 ± 0.19）。初煮NanoCerac、TI-NanoCerac、初煮ORAC和TI-ORAC的平均值分别为：0.73 ± 0.1 GAE/g茶、2.4 ± 0.70 mmol GAE/g茶、1.0 ± 0.3 mmol TE/g茶和2.1 ± 0.71 mmol TE/g茶。酿造条件，包括水温和浸泡时间显着影响抗氧化能力。在此测试的原始NanoCerac测定的高通量适应性提供了优于ORAC的优势，包括便携性和快速分析。
A novel paper-based Nanoceria Reducing Antioxidant Capacity (NanoCerac) assay for antioxidant detection, has been adapted for the first time as a high-throughput method, in order to measure the effect of brewing conditions and re-infusion on the antioxidant capacity of twenty-four commercial green teas. The oxygen radical absorbance capacity (ORAC) assay, frequently applied to complex foods and beverages, was used as a comparator measure of antioxidant capacity. A novel measure of sustained antioxidant capacity, the total inherent antioxidant capacity (TI-NanoCerac and TI-ORAC) was measured by infusing each tea six times. Effects of brewing conditions (temperature, brew time, etc.) were assessed using one popular tea as a standard. Both NanoCerac and ORAC assays correlated moderately (R2 0.80 ± 0.19). The average first-brew NanoCerac, TI-NanoCerac, first-brew ORAC and TI-ORAC were: 0.73 ± 0.1 GAE/g tea ; 2.4 ± 0.70 mmol GAE/g tea; 1.0 ± 0.3 mmol TE/g tea and 2.1 ± 0.71 mmol TE/g tea respectively. Brewing conditions including water temperature and infusion time significantly affected antioxidant capacity. The high-throughput adaptation of the original NanoCerac assay tested here offered advantages over ORAC, including portability and rapid analysis.