GABAA circuit mechanisms are associated with ether anesthesia-induced unconsciousness.
GABAA circuit mechanisms are associated with ether anesthesia-induced unconsciousness.
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DOI：
10.1016/j.clinph.2016.02.012
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发表时间：
2016-06
期刊：
影响因子：
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通讯作者：
Brown EN
中科院分区：
文献类型：
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作者：
Akeju O;Hamilos AE;Song AH;Pavone KJ;Purdon PL;Brown EN
一个新兴的范式，了解麻醉剂如何诱导改变觉醒是相关的受体靶向特定的神经回路的脑电图（EEG）活动。我们以前已经发现，增强的γ-氨基丁酸A（GABAA）抑制性突触后电流（IPSC）表现为大幅度缓慢（0.1-1 Hz）和正面相干α（8-12 Hz）振荡。因此，我们研究了现代乙醚衍生物（MDDE）的EEG特征，以评估我们可以深入了解MDDE麻醉机制的程度。我们回顾性研究了我们数据库中的病例，其中患者接受异氟烷vs.异氟烷/氯胺酮（各n=10）或地氟烷vs.地氟烷/氯胺酮（各n = 9）。我们用谱功率和相干方法分析了脑电记录。类似于已知的GABAA电路水平的机制，我们发现，MDDE诱导大幅度缓慢和正面相干α振荡。此外，MDDE还诱导了正面相干θ（4-8 Hz）振荡。用氯胺酮减少GABA能IPSC导致β/γ（13-40 Hz）振荡，并显著降低MDDE诱导的慢、θ和α振荡功率。MDDE与大振幅慢振荡和相干α和θ振荡有关。这些观察结果与GABAA回路水平机制与MDDE诱导的无意识相关的概念一致。
An emerging paradigm for understanding how anesthetics induce altered arousal is relating receptor targeting in specific neural circuits to electroencephalogram (EEG) activity. We have previously found that enhanced gamma amino-butyric acid A (GABAA) inhibitory post-synaptic currents (IPSCs) manifest with large-amplitude slow (0.1–1 Hz) and frontally coherent alpha (8–12 Hz) oscillations. Therefore, we investigated the EEG signatures of modern day derivatives of ether (MDDE) to assess the extent to which we could obtain insights into MDDE anesthetic mechanisms We retrospectively studied cases from our database in which patients received isoflurane vs. isoflurane/ketamine (n=10 each) or desflurane vs. desflurane/ketamine (n = 9 each). We analyzed the EEG recordings with spectral power and coherence methods. Similar to known GABAA circuit level mechanisms, we found that MDDE induced large amplitude slow and frontally coherent alpha oscillations. Additionally, MDDE also induced frontally coherent theta (4–8 Hz) oscillations. Reduction of GABAergic IPSCs with ketamine resulted in beta/gamma (13–40 Hz) oscillations, and significantly reduced MDDE-induced slow, theta and alpha oscillation power. MDDE are associated with large amplitude slow oscillations and coherent alpha and theta oscillations. These observations are consistent with the notion that GABAA circuit-level mechanisms are associated with MDDE-induced unconsciousness.