SINGLE-UNIT RECORDING AND STIMULATION IN SUPERIOR COLLICULUS OF ALERT RHESUS-MONKEY
SINGLE-UNIT RECORDING AND STIMULATION IN SUPERIOR COLLICULUS OF ALERT RHESUS-MONKEY
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DOI：
10.1152/jn.1972.35.6.915
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发表时间：
1972-01-01
影响因子：
2.5
通讯作者：
STRYKER, M
中科院分区：
文献类型：
--
作者：
SCHILLER, PH;STRYKER, M
方法在4只恒河猴上进行实验。其中三种动物的实验方法类似于席勒和科纳(17)所描述的方法。每只猴1只眼，分别切断第3、4、6条脑神经，在动眼的眼眶周围植入直流眼动电极。一套四个螺丝，用于随后的头部固定，和两个可调节的球窝接头，不锈钢管瞄准上端！I，被植入头骨(详情见参考文献1‘7)。管子的放置也允许进入外展神经核。在第四只猴子中，我们没有使眼睛静止。在这只动物中，研究了刺激对共轭眼球运动的影响。在记录刺激实验中，用头骨螺钉固定动物的头部(7)。被固定的眼睛的中心凹被映射到面对动物的切线屏幕上。单位活动、刺激标记和眼动活动被记录在磁带上，并被显示在多通道条形图记录器(Visicorder)上。以前面描述的方式进行眼动校准(14)。本研究的一般程序是将微电极放入上丘，记录单个单位的活动并绘制它们的感受野，然后通过相同的微电极刺激记录部位。在浅层，主要的重点是定位相对于中心凹的感受野，然后确定由电刺激引起的眼跳的大小和方向。在下层，人们遇到与眼球运动有关的细胞，比较了单个单位和电刺激获得的“运动场”(见席勒和科纳，参考文献17)。玻璃涂覆铂-铱微电极
METHODSThe experiment was carried out on four rhesus monkeys. For three of the animals the methods were similar to those described by Schiller and Koerner(17). One eye of each monkey was immobilized by transection of the 3rd, 4th, and 6th cranial nerves; dc eyemovement electrodes were implanted around the orbit of the moving eye. A set of four screws, for subsequent securing of the head, and two adjustable ball-and-socket joints, with stainless steel tubes aimed at the superior collicu! i, were implanted on the skull (details in ref 1’7). Placement of the tubes also permitted access to the abducens nucleus. In the fourth monkey we did not immobilize the eye. In this animal the effects of stimulation on conjugate eye movements were examined.During the recording-stimulation experimen ts, the animal’s head was secured using the skull screws (7). The fovea of the immobilized eye was mapped onto a tangent screen facing the animal. Unit activity, stimulation marker, and eye-movement activity were recorded on magnetic tape and were displayed on a multichannel stripchart recorder(Visicorder). Eye-movement calibration was carried out in a manner previously described (14). The general procedure in this study entailed the lowering of the microelectrode into the superior colliculus, recording the activity of single units and mapping their receptive fields, and then stimulating the recording site through the same microelectrode. In the superficial layers primary emphasis was placed on locating receptive fields relative to the fovea and then determining the size and direction of saccades elicited by electrical stimulation. In the lower layers, where one encounters cells related to eye movement, a comparison was made between the “motor fields”(see Schiller and Koerner, ref 17) obtained with single units and with electrical stimulation. Glass-coated platinum-iridium microelectrodes