Water permeability of phospholipid vesicles
Water permeability of phospholipid vesicles
复制标题
磷脂囊泡的水渗透性
DOI：
--
复制
发表时间：
1970
影响因子：
2.4
通讯作者：
R. Dowben
中科院分区：
文献类型：
--
作者：
J. Reeves;R. Dowben
概述通过跟踪悬浮液在停流装置中混合后浊度的变化来测量由一个或多个脂质双层包围的直径为0.5至10 μ的磷脂囊泡的水渗透性。提出了一种通过浊度测量来评价宽粒径范围囊泡体积变化的半经验公式。根据反应速率理论分析了流动速率。磷脂酰胆碱囊泡的水渗透系数在25°C下约为44 μ/秒，在37°C下约为70 μ/秒。水迁移的活化能为8.25千卡/摩尔。结果与水在膜中通过溶解和扩散渗透的观点一致。
SummaryThe water permeability of phospholipid vesicles 0.5 to 10 μ in diameter bounded by one or by several lipid bilayers was measured by following the change in turbidity of a suspension after mixing in a stopped flow apparatus. A semi-empirical formulation for evaluating volume changes of vesicles with a broad size range by measurement of turbidity is developed. The rate of flow is analyzed in terms of reaction rate theory. The water permeability coefficients for phosphatidylcholine vesicles were approximately 44 μ/sec at 25°C and 70 μ/sec at 37°C. The activation energy for water transport was 8.25 kcal/mole. The results were consistent with the view that water permeates by dissolution and diffusion in the membrane.