ATPase genes of diverse multidrug-resistant Acinetobacter baumannii isolates frequently harbour integrated DNA
ATPase genes of diverse multidrug-resistant Acinetobacter baumannii isolates frequently harbour integrated DNA
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多种多重耐药鲍曼不动杆菌分离株的 ATP 酶基因经常含有整合的 DNA
DOI：
10.1093/jac/dkn481
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发表时间：
2009-02-01
影响因子：
5.2
通讯作者：
Rajakumar, Kumar
中科院分区：
文献类型：
--
作者：
Shaikh, Farha;Spence, Richard P.;Rajakumar, Kumar
为了确定遗传多样性鲍曼不动杆菌分离株的ATP酶基因是否受到潜在基因组岛的干扰，采用随机扩增多态性DNA（RAPD）-PCR、序列分组和PFGE技术对50株鲍曼不动杆菌分离株的遗传多样性进行了研究。鲍曼不动杆菌分离自各种临床标本。然后使用PCR分析来鉴定具有潜在破坏的ATP酶基因的分离株。通过PCR定位和染色体步移分析ATP酶基因的侧翼区域，对代表性的不同菌株进行了进一步的鉴定，在50株供试菌株中有41株含有ATP酶基因。10个ATP酶PCR阴性代表菌株的序列组和PFGE数据证实了大量的遗传多样性。7个分离物含有末端与AbaR1的一端或两端具有高度序列相似性的元件，AbaR1是A.鲍曼不动杆菌。另一个分离物A25具有高度保守的AbaR1样3 '端，但5'端不同，尽管相关，但与A中的一个独特基因座几乎相同。鲍曼不动杆菌ATCC 17978。第九个分离株（A92）在其5 '-ATP酶残基附近拥有一个全新的序列。三个分离株似乎缺乏3'-ATP酶基因片段，与最近测序的菌株ACICU的情况相同。因此，详细研究的10个ATP酶阴性分离物中有8个具有被AbaR1样侧翼区破坏的ATP酶基因。鲍曼不动杆菌分离株经常被与AbaR1相关侧翼序列匹配的插入物破坏。然而，分离物A92的ATP酶基因被与AbaR1中发现的DNA序列不同的DNA序列破坏。
The aim of the study was to determine whether ATPase genes of genetically diverse Acinetobacter baumannii isolates are disrupted by potential genomic islands.Random amplified polymorphic DNA (RAPD)-PCR, sequence grouping and PFGE were used to investigate the genetic diversity of 50 A. baumannii isolated from various clinical specimens. PCR analysis was then used to identify isolates with a potentially disrupted ATPase gene. Representative genetically distinct isolates were further characterized by PCR mapping and chromosome walking to analyse the flanking regions of the disrupted ATPase genes.Forty-one of the 50 isolates tested appeared to contain a disrupted ATPase gene. Sequence group and PFGE data for 10 ATPase PCR-negative representative isolates confirmed substantial genetic diversity. Seven isolates contained elements with ends showing high levels of sequence similarity to one or both extremities of AbaR1, the first resistance island described in A. baumannii. A further isolate, A25, possessed a highly conserved AbaR1-like 3'-end, but a divergent, though related, 5'-terminus that exhibited near identity with a distinct locus in A. baumannii ATCC 17978. A ninth isolate (A92) possessed a completely novel sequence abutting on its 5'-ATPase remnant. Three isolates appeared to lack 3'-ATPase gene segments, as was the case with the recently sequenced strain ACICU. Thus, 8 of the 10 ATPase-negative isolates investigated in detail had ATPase genes disrupted with AbaR1-like flanking regions.ATPase genes of diverse A. baumannii isolates are frequently disrupted by insertions matching AbaR1-related flanking sequences. However, the ATPase gene of isolate A92 was disrupted by a DNA sequence distinct from those found in AbaR1.