Viral Packaging and Cell Culture for CRISPR-Based Screens.

Viral Packaging and Cell Culture for CRISPR-Based Screens.
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用于基于 CRISPR 筛选的病毒包装和细胞培养。

DOI:
10.1101/pdb.prot090811
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发表时间:
2016
影响因子:
--
通讯作者:
Sabatini,DavidM
Sabatini,DavidM
中科院分区:
--
文献类型:
--
作者:
Wang,Tim;Lander,EricS;Sabatini,DavidM

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方法注:您必须联系您所在机构的生物安全办公室,以获得机构特定的说明。您必须遵循安全程序,并在适合处理慢病毒的环境(例如BL 2+)中工作。病毒包装的一般概述可以在这里找到:https://www.添加基因。org/lentiviral/packaging/.
MethodNOTE: You must contact your institution’s Bio-Safety office to receive institution-specific instructions. You must follow safety procedures and work in an environment (eg BL2+) suitable for handling lentiviruses. A general overview of viral packaging can be found here: https://www. addgene. org/lentiviral/packaging/.
DOI: 10.1101/pdb.prot090803
发表时间: 2016-03-01
影响因子: --
作者:
Wang T;Lander ES;Sabatini DM
通讯作者: Sabatini DM