Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode
Chemotaxonomy of bacteria by comprehensive GC and GC-MS in electron impact and chemical ionisation mode
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DOI：
10.1002/jssc.200800215
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发表时间：
2008-10-01
影响因子：
3.1
通讯作者：
Sandra, Pat
中科院分区：
文献类型：
--
作者：
David, Frank;Tienpont, Bart;Sandra, Pat
描述了细菌的细胞外部分的分析。水解和甲基化后，脂肪酸甲酯（FAME）通过使用Sherlock细菌鉴定系统的1-D GC、通过综合GC（GC x GC）以及通过电子轰击（EI）和正化学电离（PCI）模式的GC-MS来测定。通过GC x GC，增强的选择性和基团类型分离提供了微生物中脂肪酸的更完整的解析。GC-EI-MS和GC-PCI-MS有助于确认。本研究中选择的细菌是缺陷短波单胞菌、金黄杆菌和嗜麦芽窄食单胞菌。
The analysis of the cellular lipidic fraction of bacteria is described. After hydrolysis and methylation, the fatty acid methyl esters (FAMEs) are determined by 1-D GC using the Sherlock MIDI bacteria identification system, by comprehensive GC (GC x GC) and by GC-MS in electron impact (EI) and positive chemical ionisation (PCI) mode. With GC x GC, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. GC-EI-MS and GC-PCI-MS were helpful for confirmation. The bacteria selected in this study are Brevundimonas diminuta, Chryseobacterium gleum and Stenotrophomonas maltophilia.