Investigation of the Diels-Alder reaction as a cross-linking mechanism for degradable poly(ethylene glycol) based hydrogels
Investigation of the Diels-Alder reaction as a cross-linking mechanism for degradable poly(ethylene glycol) based hydrogels
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DOI：
10.1039/c3tb20831a
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发表时间：
2013-01-01
影响因子：
7
通讯作者：
Goepferich, Achim M.
中科院分区：
文献类型：
--
作者：
Kirchhof, Susanne;Brandl, Ferdinand P.;Goepferich, Achim M.
研究了Diels-Alder（DA）反应作为聚乙二醇（PEG）基水凝胶的交联机理。通过用呋喃基和马来酰亚胺基官能化星形聚乙二醇，合成了两种互补的大分子单体。凝胶形成在37 ° C的水中发生;取决于所使用的水凝胶制剂，凝胶化时间在171 +/-25分钟和14 +/-1分钟之间。复合剪切模量取决于大分子单体的浓度、支化因子和分子量;观察到的值在2821 +/- 1479 Pa和37097 +/- 6698 Pa之间。水凝胶的溶胀和降解受到相同参数的影响;降解时间在几天到几周之间变化。凝胶溶解被发现发生逆DA反应和随后的马来酰亚胺基团的水解。网络网孔大小的计算表明，所制备的水凝胶将适合于治疗性蛋白质的控制释放。
The Diels-Alder (DA) reaction was investigated as a cross-linking mechanism for poly(ethylene glycol) (PEG) based hydrogels. Two complementary macromonomers were synthesized by functionalizing star-shaped PEG with furyl and maleimide groups. Gel formation occurred in water at 37 degrees C; the gelation time ranged between 171 +/- 25 min and 14 +/- 1 min depending on the used hydrogel formulation. The complex shear modulus was dependent on the concentration, branching factor and molecular weight of the macromonomers; values between 2821 +/- 1479 Pa and 37097 +/- 6698 Pa were observed. Hydrogel swelling and degradation were influenced by the same parameters; the degradation time varied between a few days and several weeks. Gel dissolution was found to occur by retro-DA reaction and subsequent hydrolysis of maleimide groups. Calculations of the network mesh size revealed that the prepared hydrogels would be suitable for the controlled release of therapeutic proteins.