Prenatal development of peptidergic primary afferent projections to mouse lumbosacral autonomic preganglionic cell columns
Prenatal development of peptidergic primary afferent projections to mouse lumbosacral autonomic preganglionic cell columns
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DOI：
10.1016/s0165-3806(03)00165-2
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发表时间：
2003-08-12
期刊：
影响因子：
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通讯作者：
Kishida, R
中科院分区：
文献类型：
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作者：
Funakoshi, K;Goris, RC;Kishida, R
为了检查脊髓内脏反射的产前发育，在脊髓中，特别是在小鼠胚胎的自主神经节前核中检查了降钙素基因相关肽（CGRP）免疫反应的初级感觉神经纤维。胚胎第16天（E16）。CGRP免疫反应纤维首先观察到在骶骨中间外侧核（IML）的副交感神经司以及在腰中央自主神经核（CA）的交感神经司，在那里他们出现在接近节前神经元胞体胆碱乙酰转移酶或一氧化氮合酶免疫反应。大多数CGRP免疫反应阳性的静脉曲张是阴性的P物质。P物质免疫反应阳性的静脉曲张散在这些核，但没有看到对节前神经元胞体的沉积。在E18，CGRP免疫反应纤维在骶骨IML和腰椎CA更丰富。在E18，在非常接近节前神经元胞体的静脉曲张中经常观察到P物质和CGRP的共表达。CGRP免疫反应纤维也观察到在腰椎IML E18，虽然显着较少，发现在这个核与骶骨IML相比。与上腰椎水平相反，在胸水平的IML中未观察到CGRP免疫反应性纤维。这些结果表明，肽能初级感觉纤维的生长项目的自主节前神经元在胚胎时期的选择性目标。肽能初级感觉纤维和支配盆腔内脏的节前神经元之间潜在的直接联系可能为胚胎脊髓内脏反射活动提供了一个回路。(C)2003 Elsevier B.V.保留所有权利。
To examine the prenatal development of spinal visceral reflexes, primary sensory nerve fibers immunoreactive for calcitonin gene-related peptide (CGRP) were examined in the spinal cord, particularly in the autonomic preganglionic nuclei of mouse embryos. On embryonic day 16 (E16). CGRP-immunoreactive fibers were first observed in the sacral intermediolateral nucleus (IML) of the parasympathetic division as well as in the lumbar central autonomic nucleus (CA) of the sympathetic division, where they appeared in proximity to preganglionic neuronal perikarya immunoreactive for choline acetyltransferase or nitric oxide synthase. Most of the CGRP-immunoreactive varicosities were negative for substance P. Substance P-immunoreactive varicosities were scattered in these nuclei, but no appositions were seen on the preganglionic neuronal perikarya. On E18, CGRP-immunoreactive fibers were more abundant in the sacral IML and the lumbar CA. Co-expression of substance P and CGRP was frequently observed in the varicosities very close to the preganglionic neuronal perikarya on E18. CGRP-immunoreactive fibers were also observed in the lumbar IML on E18, although significantly fewer were found in this nucleus compared with the sacral IML. In contrast to the upper lumbar level, no fibers immunoreactive for CGRP were observed in the IML at the thoracic level. These results suggest that peptidergic primary sensory fibers grow to project to the selective targets of autonomic preganglionic neurons during the embryonic period. The potential direct connections between the peptidergic primary sensory fibers and preganglionic neurons innervating the pelvic viscera might provide a circuit for spinal visceral reflexes active in embryos. (C) 2003 Elsevier B.V. All rights reserved.