The effect of topical drugs on mouse ear epidermal transglutaminase activity.
The effect of topical drugs on mouse ear epidermal transglutaminase activity.
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局部药物对小鼠耳表皮转谷氨酰胺酶活性的影响。
DOI：
10.1111/1523-1747.ep12500054
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
S. Ballaron
中科院分区：
文献类型：
--
作者：
L. D. De Young;S. Ballaron
本文测定了小鼠耳表皮谷氨酰胺转氨酶对单次应用蒽啉、维甲酸(59微克/耳)或氟喹诺酮丙酮(2微克/耳)的反应。安曲林和维甲酸引起炎症和表皮蛋白质和DNA的积聚，而氟喹诺酮则导致耳朵变薄，表皮蛋白质和DNA减少。无论是用没食子酸还是维甲酸治疗，每只耳朵的表皮转谷氨酰胺酶的绝对量都会增加。从48小时到7天，安曲林使这一参数比丙酮处理的对照组增加了70%-100%。维甲酸处理的耳朵最初表现出较慢的增长，但在96小时时达到峰值，是对照水平的4倍，然后在7天后恢复正常。氟喹诺酮处理对此参数无影响。表皮转谷氨酰胺酶(总表皮转谷氨酰胺酶/总可溶性表皮蛋白)的比活力在维甲酸处理后降低；在7天时，除7天时下降50%外，均维持在正常水平；并被氟喹诺酮丙酮显著刺激。在后一种情况下，比活性在96小时时超过对照的5倍，并在7天时仍接近这一水平。表皮谷氨酰胺转氨酶活性是分化的标志，蛋白质和DNA的积累是生长的标志。因此，在所研究的剂量下，维甲酸有利于生长而不是分化，苯甲酸保持正常或接近正常的生长与分化的比率，而氟喹诺酮强烈促进分化而不是生长。
The response of mouse ear epidermal transglutaminase to single applications of anthralin, retinoic acid (both 59 micrograms/ear) or fluocinolone acetonide (2 micrograms/ear) was determined. Anthralin and retinoic acid caused inflammation and accumulation of epidermal protein and DNA, whereas fluocinolone acetonide resulted in ear thinning and decreased epidermal protein and DNA. Treatment with either anthralin or retinoic acid caused increases in absolute amounts of epidermal transglutaminase activity/ear. Anthralin increased this parameter 70-100% above acetone-treated controls from 48 hr through 7 days. Retinoic acid-treated ears showed a slower initial increase but peaked at 4 times control level by 96 hr before returning to normal at 7 days. Fluocinolone acetonide treatment had no effect on this parameter. The specific activity of epidermal transglutaminase (total epidermal transglutaminase/total soluble epidermal protein) was decreased by retinoic acid treatment; was maintained at normal levels by anthralin (except for the 7-day point where it decreased 50%); and was dramatically stimulated by fluocinolone acetonide. In the latter case, specific activity was more than 5 x control by 96 hr and still near this level at 7 days. Epidermal transglutaminase activity is a marker of differentiation, and protein and DNA accumulation an indication of growth. Thus, at the doses studied, retinoic acid favors growth over differentiation, anthralin maintains a normal to near normal ratio of growth to differentiation, and fluocinolone acetonide strongly favors differentiation over growth.