Pteridine formation during lectin‐induced lymphocyte activation
Pteridine formation during lectin‐induced lymphocyte activation
复制标题
凝集素诱导的淋巴细胞活化过程中蝶啶的形成
DOI：
10.1002/jcb.240280303
复制
发表时间：
1985
影响因子：
4
通讯作者：
I. Ziegler
中科院分区：
文献类型：
--
作者：
I. Ziegler
碘氧化后，通过反相HPLC、Crithidia试验和氧化降解在小鼠脾淋巴细胞中鉴别出生物蝶呤、6-羟甲基蝶呤和6-甲酰蝶呤。伴刀豆球蛋白A的激活导致蝶啶的量增加30倍;生物蝶呤以及甲醇和醛的总和达到6-8 × 10−12 mol/106个细胞的水平。最快的增加发生在前24小时。因此，蝶啶积累之前的淋巴细胞增殖期;最大的DNA合成后发现72小时。生物蝶呤主要保留在细胞内，而6-羟甲基蝶呤和6-甲酰蝶呤被发现在上清液中，如果刺激的细胞随后在磷酸盐缓冲盐溶液（PBS）中孵育。在对照细胞的PBS上清液中发现了异黄蝶呤，所述对照细胞先前仅保持在培养基中而不是经受凝集素刺激。在这些细胞内仅发现极少量的蝶呤，并且这种蝶呤在刺激的淋巴细胞中不存在。细胞蝶啶的早期增加及其差异释放很可能为其对白细胞介素-2活性的调节作用提供了基础（齐格勒I，et al：Apriphokine Research 3：284，1984）。
After iodine oxidation, biopterin, 6‐hydroxymethylpterin, and 6‐formylpterin were identified in mouse spleen lymphocytes by means of reverse‐phase HPLC, Crithidia assay, and oxidative degradation. Concanavalin A activation induces a 30‐fold increase in the pteridine amounts; biopterin as well as the sum of the carbinol and the aldehyde attain levels of 6–8 × 10−12 mol/106 cells. The most rapid increase occurs during the first 24 hr. Thus, pteridine accumulation precedes the period of lymphocyte proliferation; maximum DNA synthesis was found after 72 hr. Biopterin remains largely inside the cells, whereas 6‐hydroxymethylpterin and 6‐formylpterin were found in the supernatant if the stimulated cells were subsequently incubated in a phosphate buffered salt solution (PBS). Isoxanthopterin was found in the PBS supernatant of control cells that previously were kept in medium alone rather than subjected to lectin stimulation. Only minimal amounts were found inside these cells, and this pterin was absent from the stimulated lymphocytes. The early increase in cellular pteridines and their differential release may well provide the basis for their modulating effect on interleukin‐2 activity (Ziegler I, et al: Lymphokine Research 3:284, 1984).