Circular RNA and Messenger RNA Expression Profile and Competing Endogenous RNA Network in Subchondral Bone in Osteonecrosis of the Femoral Head
Circular RNA and Messenger RNA Expression Profile and Competing Endogenous RNA Network in Subchondral Bone in Osteonecrosis of the Femoral Head
复制标题
股骨头坏死软骨下骨中的环状 RNA 和信使 RNA 表达谱及竞争性内源性 RNA 网络
DOI：
10.1089/dna.2020.5894
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发表时间：
2020-11-13
影响因子：
3.1
通讯作者：
Yang, Pei
中科院分区：
文献类型：
--
作者：
Jiao, Ming;Tian, Run;Yang, Pei
股骨头坏死（Osteonecrosis of femoral head， ONFH）是一种常见的破坏性骨科疾病，其发病机制尚不明确。关于环状rna （circRNAs）在ONFH软骨下骨中的作用的研究有限。本研究旨在通过下一代测序分析ONFH患者软骨下骨中环状rna和信使rna （mrna）的差异表达，并通过生物信息学分析探索这些分子在ONFH中的潜在调控关系。结果，我们检测到74个异常表达的环状rna和121个差异表达的mrna。基因本体和京都基因与基因组百科全书路径富集分析表明，成骨细胞和破骨细胞对成骨能力的影响主要与几个重要的生物学过程和信号通路有关。此外，我们尝试构建与57个异常基因的蛋白-蛋白相互作用网络和与3个选定的circrna竞争的内源RNA网络，初步揭示了这些分子在ONFH中的调控作用及其相互关系。此外，实时定量PCR验证了我们网络中circrna与ONFH分子机制的潜在关联。总之，我们的研究结果可能促进对ONFH机制的理解，并为ONFH的早期诊断和干预提供新的见解。
Osteonecrosis of the femoral head (ONFH) is a common and destructive orthopedic disease, of which the pathogenesis mechanism remains elusive. Limited studies have been conducted to investigate the role of circular RNAs (circRNAs) in subchondral bone in ONFH. This study aimed to profile differential expression of circRNAs and messenger RNAs (mRNAs) in subchondral bone obtained from ONFH patients by next-generation sequencing, and explore the potential regulatory relationship of these molecules in ONFH by bioinformatics analysis. As a result, we detected 74 aberrantly expressed circRNAs and 121 differentially expressed mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated several vital biological processes and signaling pathways, which are primarily related to osteogenic capacity influenced by osteoblasts and osteoclasts. Furthermore, attempting construction of protein-protein interaction network with 57 aberrant genes and competing endogenous RNA network with 3 selected circRNAs preliminarily revealed the regulatory roles and the relationships of these molecules in ONFH. In addition, the potential association of circRNAs in our networks with the molecular mechanism of ONFH was validated by real time-quantitative PCR. In conclusion, our findings may promote understanding the mechanism of ONFH, and offer a novel insight into early diagnosis and intervention of ONFH.