Polyamines Regulate Strawberry Fruit Ripening by Abscisic Acid, Auxin, and Ethylene
Polyamines Regulate Strawberry Fruit Ripening by Abscisic Acid, Auxin, and Ethylene
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多胺通过 ABA、IAA 和乙烯调节草莓果实成熟
DOI：
10.1104/pp.18.00245
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发表时间：
2018-05-01
期刊：
影响因子：
7.4
通讯作者：
Shen, Yuanyue
中科院分区：
文献类型：
--
作者：
Guo, Jiaxuan;Wang, Shufang;Shen, Yuanyue
多胺（Polyamines，PAs）参与植物的生长发育过程，包括果实的成熟。然而，目前尚不清楚PA是否在草莓（草莓）成熟中发挥作用，模式nonclimacteric植物。在这里，我们发现，PA精胺（Spm）的含量增加更急剧的发病后，果实着色比PA腐胺（Put）或亚精胺（Spd）。成熟果实中的SPM优势来自草莓S-腺苷-L-Met脱羧酶基因（FaSAMDC）的丰富转录本，该基因编码产生PA生物合成所需的残基的酶。外源Spm和Spd促进果实着色，而外源Put和SAMDC抑制剂抑制着色。基于转录组数据，上调和下调FaSAMDC表达分别促进和抑制成熟，这与几个生理参数及其相应的基因转录的变化相一致，包括硬度，花青素含量，糖含量，多胺含量，生长素（吲哚-3-乙酸[IAA]）含量，脱落酸（阿坝）含量和乙烯释放。用等温滴定量热法测得FaSAMDC也具有较高的酶活性，其Kd值为1.7 ± 3 × 10（-3）M。总之，PAs，特别是Spm，调节草莓果实成熟ABA主导，IAA参与，乙烯协调的方式，和FaSAMDC起着重要的作用。
Polyamines (PAs) participate in many plant growth and developmental processes, including fruit ripening. However, it is not clear whether PAs play a role in the ripening of strawberry (Fragaria ananassa), a model nonclimacteric plant. Here, we found that the content of the PA spermine (Spm) increased more sharply after the onset of fruit coloration than did that of the PAs putrescine (Put) or spermidine (Spd). Spm dominance in ripe fruit resulted from abundant transcripts of a strawberry S-adenosyl-L-Met decarboxylase gene (FaSAMDC), which encodes an enzyme that generates a residue needed for PA biosynthesis. Exogenous Spm and Spd promoted fruit coloration, while exogenous Put and a SAMDC inhibitor inhibited coloration. Based on transcriptome data, up- and down-regulation of FaSAMDC expression promoted and inhibited ripening, respectively, which coincided with changes in several physiological parameters and their corresponding gene transcripts, including firmness, anthocyanin content, sugar content, polyamine content, auxin (indole-3-acetic acid [IAA]) content, abscisic acid (ABA) content, and ethylene emission. Using isothermal titration calorimetry, we found that FaSAMDC also had a high enzymatic activity with a K-d of 1.7 3 10(-3) M. In conclusion, PAs, especially Spm, regulate strawberry fruit ripening in an ABA-dominated, IAA-participating, and ethylene-coordinated manner, and FaSAMDC plays an important role in ripening.