Chemical and Physical Modulation of Antibiotic Activity in Emericella Species
Chemical and Physical Modulation of Antibiotic Activity in Emericella Species
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DOI：
10.1002/cbdv.201100362
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发表时间：
2012-06-01
影响因子：
2.9
通讯作者：
Bills, Gerald F.
中科院分区：
文献类型：
--
作者：
de la Cruz, Mercedes;Martin, Jesus;Bills, Gerald F.
在培养基和固态发酵中添加表观遗传修饰剂和离子交换树脂已被促进为刺激潜在生物合成基因簇表达和调节次级代谢物生物合成的方式。我们询问了这些处理的组合将如何影响筛选分离株的群体及其相对于发酵对照的发酵模式。一组43 Emericella菌株，代表25个物种和品种，生长在营养丰富的培养基，包括葡萄糖，酪蛋白水解产物，尿素，和矿物盐。每种菌株在未处理的搅拌液体培养基中生长，用辛二酰苯胺异羟肟酸、组蛋白脱乙酰酶抑制剂、5-氮杂胞苷、DNA甲基化抑制剂、Amberlite非离子聚丙烯酸酯树脂处理的培养基，以及掺入惰性静态蛭石基质中的相同培养基。物种固有的代谢差异更强烈地影响模式的pesticsis比中等治疗。抗菌铁载体，去铁三乙酰fusigen（1），在大多数物种在液体培养基中检测到，但不是在蛭石介质。检测到的主要抗真菌成分为棘白菌素B。一些物种产生这种抗真菌剂，无论治疗，虽然较高的数量往往产生蛭石。首次报道了几个物种产生棘白菌素B（3）。从E. quadrilineata。
The addition of epigenetic modifying agents and ion-exchange resins to culture media and solid-state fermentations have been promoted as ways to stimulate expression of latent biosynthetic gene clusters and to modulate secondary metabolite biosynthesis. We asked how combination of these treatments would affect a population of screening isolates and their patterns of antibiosis relative to fermentation controls. A set of 43 Emericella strains, representing 25 species and varieties, were grown on a nutrient-rich medium comprising glucose, casein hydrolysate, urea, and mineral salts. Each strain was grown in untreated agitated liquid medium, a medium treated with suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, 5-azacytidine, a DNA methylation inhibitor, an Amberlite non-ionic polyacrylate resin, and the same medium incorporated into an inert static vermiculite matrix. Species-inherent metabolic differences more strongly influenced patterns of antibiosis than medium treatments. The antibacterial siderophore, desferritriacetylfusigen (1), was detected in most species in liquid media, but not in the vermiculite medium. The predominant antifungal component detected was echinocandin B. Some species produced this antifungal regardless of treatment, although higher quantities were often produced in vermiculite. Several species are reported for the first time to produce echinocandin B (3). A new echinocandin analog, echinocandin E (2), was identified from E. quadrilineata.