Effects of K+-stimulation and precursor loading on the in vivo release of dopamine, serotonin and their metabolites in the nucleus accumbens of the rat.
Effects of K+-stimulation and precursor loading on the in vivo release of dopamine, serotonin and their metabolites in the nucleus accumbens of the rat.
复制标题
钾刺激和前体负荷对大鼠伏核体内多巴胺、血清素及其代谢物释放的影响。
DOI：
10.1016/0024-3205(87)90081-6
复制
发表时间：
1987
期刊：
影响因子：
6.1
通讯作者：
McBride,WJ
中科院分区：
文献类型：
--
作者：
Guan,XM;McBride,WJ
用推拉式套管法研究了麻醉大鼠延髓核内源性3，4-二羟基苯乙胺（DA）、5-羟色胺（5-HT）、3，4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）和5-羟吲哚乙酸（5-HIAA）的体内外排。用35 mM K+局部灌注10分钟，（P< 0.01）增加DA和5-HT的释放，但对它们的代谢产物无明显影响，从其各自的对照水平0.95和0.04 pmol/15 min至2.5和0.23 pmol/15 min。第二次和第三次导致DA和5-HT的刺激释放量减少。在每次去极化前30分钟开始，用50 μ M L-酪氨酸和色氨酸局部灌注10分钟，可以防止这种降低。在灌注液中观察到的DOPAC，HVA和5-HIAA的基线量比5-HT和DA的基础水平高出数倍。在不存在前体的情况下，DOPAC，HVA和5-HIAA的外排分别从第一次收集到最后一次收集的基线分数下降约60%，40%和25%。添加前体阻止DOPAC和5-HIAA的降低，但不能阻止HVA的降低。数据表明，（a）DA和5-HT的体内释放，沿着其代谢产物，可以同时测量与本程序，和（B）当使用推拉套管，局部灌注前体可能是必要的持续和/或重复的刺激期后，以补充单胺递质池。
The in vivo efflux of endogenous 3, 4-dihydroxyphenylethylamine (DA) 5-hydroxytryptamine (5-HT), 3, 4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) in the nucleus accumbens of the anesthetized rat was studied using a push-pull cannula. Local perfusion for 10 minutes with 35 mM K+ significantly (P< 0.01) increased the release of DA and 5-HT, but not their metabolites, from their respective control levels of 0.95 and 0.04 pmol/15 min to 2.5 and 0.23 pmol/15 min. Exposure to 35 mM K+ a second and third time resulted in a decrement in the amount of stimulated release for both DA and 5-HT. This decrease was prevented by local perfusion for 10 minutes with 50 uM L-tyrosine and-tryptophan starting 30 minutes before each episode of depolarization. The baseline amounts of DOPAC, HVA and 5-HIAA observed in the perfusates were several fold higher than the basal levels found for 5-HT and Da. In the absence of precursors, the efflux of DOPAC, HVA and 5-HIAA decreased approximately 60, 40 and 25%, respectively, from the first to the last baseline fraction collected. Addition of precursors prevented the decrease for DOPAC and 5-HIAA but not for HVA. The data indicated that (a) the in vivo release of DA and 5-HT, along with their metabolites, could be simultaneously measured with the present procedure, and (b) when using the push-pull cannula, local perfusion with precursors may be necessary following periods of sustained and/or repeated stimulation in order to replenish the monoamine transmitter pools.